Age-related changes in phagocytic activity and production of pro-inflammatory cytokines by lipopolysaccharide stimulated porcine alveolar macrophages

Islam, Mohammad Ariful, Uddin, Muhammad Jasim, Tholen, Ernst, Tesfaye, Dawit, Looft, Christian, Schellander, Karl and Cinar, Mehmet Ulas (2012) Age-related changes in phagocytic activity and production of pro-inflammatory cytokines by lipopolysaccharide stimulated porcine alveolar macrophages. Cytokine, 60 3: 707-717. doi:10.1016/j.cyto.2012.08.011


Author Islam, Mohammad Ariful
Uddin, Muhammad Jasim
Tholen, Ernst
Tesfaye, Dawit
Looft, Christian
Schellander, Karl
Cinar, Mehmet Ulas
Title Age-related changes in phagocytic activity and production of pro-inflammatory cytokines by lipopolysaccharide stimulated porcine alveolar macrophages
Journal name Cytokine   Check publisher's open access policy
ISSN 1043-4666
Publication date 2012-12
Sub-type Article (original research)
DOI 10.1016/j.cyto.2012.08.011
Volume 60
Issue 3
Start page 707
End page 717
Total pages 11
Place of publication London, United Kingdom
Publisher Academic Press
Language eng
Abstract The aim of the present study was to determine the age-related changes of phagocytic capacity and the kinetic production of cytokines in lipopolysaccharide-stimulated porcine alveolar macrophages. For this purpose, AMs were isolated from 5 (newborn), 40 (post-weaned) and 120 (young) day old pigs. Results of phagocytosis assay showed that AMs from newborn piglets had less phagocytic capacity than those of young pigs (p<0.05). For the kinetics study, cells and supernatant were collected at 1, 6, 12, 24, 36 and 48 h after LPS stimulation for quantification of cytokine mRNA and protein by quantitative real-time PCR and ELISA, respectively. The kinetics results showed that AMs from newborn piglets were significantly less capable of producing IL1β, IL6, IL12β, TNFα and IL8 than post-weaned piglets or young pigs. IL18 mRNA did not show significant differences between ages. MIP2 and MCP1 mRNA was higher in young pigs. Hence, higher production of cytokines by AMs may be the surfactant factors in the pulmonary host defense system. These results indicate that AMs from newborn piglets might be functionally immature, which may lead to increased susceptibility to lung infections. Future studies of cytokine kinetics in more animals are clearly needed to confirm these results across a wider age range.
Keyword Age-dependent
Alveolar macrophage
Phagocytosis
Lipopolysaccharide
Cytokine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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