Origin of Langerhans cells in normal skin and chronic GVHD after hematopoietic stem cell transplantation

Andani, Rafiq, Robertson, Ivan, MacDonald, Kelli P. A., Durrant, Simon, Hill, Geoffrey R. and Khosrotehrani, Kiarash (2013) Origin of Langerhans cells in normal skin and chronic GVHD after hematopoietic stem cell transplantation. Experimental Dermatology, Accepted Article 1-11. doi:10.1111/exd.12301

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Andani, Rafiq
Robertson, Ivan
MacDonald, Kelli P. A.
Durrant, Simon
Hill, Geoffrey R.
Khosrotehrani, Kiarash
Title Origin of Langerhans cells in normal skin and chronic GVHD after hematopoietic stem cell transplantation
Journal name Experimental Dermatology   Check publisher's open access policy
ISSN 0906-6705
1600-0625
Publication date 2013-12-07
Year available 2013
Sub-type Article (original research)
DOI 10.1111/exd.12301
Open Access Status
Volume Accepted Article
Start page 1
End page 11
Total pages 11
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2014
Language eng
Formatted abstract
Chronic Graft vs. Host Disease (cGVHD) is a common complication following allogeneic stem cell transplantation (SCT). Past studies have implicated the persistence of host antigen presenting cells (APCs) in GVHD. Our objective was to determine the frequency of host Langerhans cells (LCs) in normal skin post SCT and ask if their persistence could predict cGVHD.

Biopsies of normal skin from 124 sex-mismatched T cell replete allogenic SCT recipients were taken 100 days post-transplant. Patients with acute GVHD and those with less than 9 months of follow-up were excluded and prospective follow-up information was collected from remaining 22 patients. CD1a staining and X&Y chromosome in-situ hybridization were performed to label LCs and to identify their host or donor origin.

At 3 months 59±5% of LCs were host derived. The density of LCs and the proportion of host derived LCs was similar between patients that did or did not develop cGVHD.

Most LCs in the skin remained of host origin 3 months after SCT regardless of cGVHD status. This finding is in line with the redundant role of LCs in acute GVHD initiation uncovered in recent experimental models.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Accepted manuscript online: 7 DEC 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 10 Dec 2013, 14:20:04 EST by Roheen Gill on behalf of UQ Centre for Clinical Research