Venomics of new world pit vipers: Genus-wide comparisons of venom proteomes across agkistrodon

Lomonte, Bruno, Tsai, Wan-Chih, Urena-Diaz, Juan Manuel, Sanz, Libia, Mora-Obando, Diana, Sanchez, Elda E., Fry, Bryan G., Gutierrez, Jose Maria, Gibbs, H. Lisle, Sovic, Michael G. and Calvete. Juan J. (2014) Venomics of new world pit vipers: Genus-wide comparisons of venom proteomes across agkistrodon. Journal of Proteomics, 96 103-116. doi:10.1016/j.jprot.2013.10.036


Author Lomonte, Bruno
Tsai, Wan-Chih
Urena-Diaz, Juan Manuel
Sanz, Libia
Mora-Obando, Diana
Sanchez, Elda E.
Fry, Bryan G.
Gutierrez, Jose Maria
Gibbs, H. Lisle
Sovic, Michael G.
Calvete. Juan J.
Title Venomics of new world pit vipers: Genus-wide comparisons of venom proteomes across agkistrodon
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2014-01-16
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jprot.2013.10.036
Open Access Status
Volume 96
Start page 103
End page 116
Total pages 14
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Collection year 2014
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
Abstract We report a genus-wide comparison of venom proteome variation across New World pit vipers in the genus Agkistrodon. Despite the wide variety of habitats occupied by this genus and that all its taxa feed on diverse species of vertebrates and invertebrate prey, the venom proteomes of copperheads, cottonmouths, and cantils are remarkably similar, both in the type and relative abundance of their different toxin families. The venoms from all the eleven species and subspecies sampled showed relatively similar proteolytic and PLA2 activities. In contrast, quantitative differences were observed in hemorrhagic and myotoxic activities in mice. The highest myotoxic activity was observed with the venoms of A. b. bilineatus, followed by A. p. piscivorus, whereas the venoms of A. c. contortrix and A. p. leucostoma induced the lowest myotoxic activity. The venoms of Agkistrodon bilineatus subspecies showed the highest hemorrhagic activity and A. c. contortrix the lowest. Compositional and toxicological analyses agree with clinical observations of envenomations by Agkistrodon in the USA and Central America. A comparative analysis of Agkistrodon shows that venom divergence tracks phylogeny of this genus to a greater extent than in Sistrurus rattlesnakes, suggesting that the distinct natural histories of Agkistrodon and Sistrurus clades may have played a key role in molding the patterns of evolution of their venom protein genes. Biological significance: A deep understanding of the structural and functional profiles of venoms and of the principles governing the evolution of venomous systems is a goal of venomics. Isolated proteomics analyses have been conducted on venoms from many species of vipers and pit vipers. However, making sense of these large inventories of data requires the integration of this information across multiple species to identify evolutionary and ecological trends. Our genus-wide venomics study provides a comprehensive overview of the toxic arsenal across Agkistrodon and a ground for understanding the natural histories of, and clinical observations of envenomations by, species of this genus.
Keyword Genus Agkistrodon
Mass spectrometry
Snake venomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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