Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model

Goh, Lucas Y. H., Hobson-Peters Jody, Prow, Natalie A., Gardner, Joy, Bielefeldt-Ohmann, Helle, Pyke, Alyssa T., Suhrbier, Andreas and Hall, Roy A. (2013) Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model. Clinical Immunology, 149 PB: 487-497. doi:10.1016/j.clim.2013.10.004


Author Goh, Lucas Y. H.
Hobson-Peters Jody
Prow, Natalie A.
Gardner, Joy
Bielefeldt-Ohmann, Helle
Pyke, Alyssa T.
Suhrbier, Andreas
Hall, Roy A.
Title Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model
Journal name Clinical Immunology   Check publisher's open access policy
ISSN 1521-6616
1521-7035
Publication date 2013
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.clim.2013.10.004
Volume 149
Issue PB
Start page 487
End page 497
Total pages 11
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2014
Language eng
Subject 2403 Immunology
2723 Immunology and Allergy
Abstract Chikungunya virus (CHIKV) recently caused the largest epidemic ever recorded for this virus involving an estimated 1.4-6.5. million cases, with imported cased reported in over 40 countries. The number of monoclonal antibodies specific for this re-emerging alphavirus is currently limited. Herein we describe the generation and characterisation of five monoclonal antibodies specific for the E2 glycoprotein of CHIKV. The antibodies detected a range of CHIKV isolates in several assays including ELISA, Western blot, immunofluorescence assay (IFA) and immunohistochemistry (IHC) without evidence of cross-reactivity with other alphaviruses. Four antibodies also neutralised CHIKV in vitro, two of which provided complete protection against arthritis in a CHIKV mouse model when administered prior to infection. Given the current shortage of widely available reagents for CHIKV, these specific antibodies will be useful not only in research, but may also provide the basis for new diagnostics and treatments.
Keyword Chikungunya virus
Diagnostic
Disease protection
ELISA
Immunohistochemistry
Monoclonal antibodies
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
School of Veterinary Science Publications
 
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