Putative cancer stem cell marker expression in oral epithelial dysplasia and squamous cell carcinoma

Abdulmajeed, A. A., Dalley, A. J. and Farah, C. S. (2013) Putative cancer stem cell marker expression in oral epithelial dysplasia and squamous cell carcinoma. Journal of Oral Pathology and Medicine, 42 10: 755-760. doi:10.1111/jop.12073


Author Abdulmajeed, A. A.
Dalley, A. J.
Farah, C. S.
Title Putative cancer stem cell marker expression in oral epithelial dysplasia and squamous cell carcinoma
Journal name Journal of Oral Pathology and Medicine   Check publisher's open access policy
ISSN 0904-2512
1600-0714
Publication date 2013-11
Year available 2013
Sub-type Article (original research)
DOI 10.1111/jop.12073
Volume 42
Issue 10
Start page 755
End page 760
Total pages 6
Place of publication Wiley-Blackwell Publishing
Publisher West Sussex, United Kingdom
Collection year 2014
Language eng
Subject 1306 Cancer Research
2734 Pathology and Forensic Medicine
2733 Otorhinolaryngology
3504 Oral Surgery
3506 Periodontics
Abstract Multifactorial conditions underlie progression of potentially malignant oral lesions (PMOL) to oral squamous cell carcinoma (OSCC) and there is currently need for better prediction of malignant transformation. The hypothesised existence of cancer stem cells in dysplastic oral tissues provides the potential for more informed assessment of PMOL progression. Semi-quantitative immunohistochemical assessment of four putative cancer stem cell markers (CD24, CD44, CD271 and ALDH1) was conducted with a training cohort of 107 patient biopsies to establish clinically applicable score threshold values that were subsequently applied to a blind diagnosis in an independent validation cohort of 278 biopsies. Stain intensity scores for ALDH1, CD24 and CD44, but not CD271 were greater for OSCC than normal tissues. The intensity of ALDH1 and CD24 immunostaining correlated with increased oral epithelial disease severity, and CD24 was effective in distinguishing OSCC from non-malignant tissues, correctly diagnosing 71% of OSCC cases in the validation cohort. Importantly, CD24 immunostaining was effective in diagnosing the presence of dysplasia, correctly discriminating 69% of dysplasia tissues from normal tissues, although no distinction between mild and severe grades of dysplasia was achieved. The results highlight CD24 immunostain intensity as an effective marker of oral dysplasia and OSCC. In conclusion, CD24 immunostain intensity scoring may serve as a helpful technique to assist with the histological recognition of dysplasia in oral biopsies, but not for distinguishing between grades of dysplasia.
Keyword ALDH1
CD24
CD271
CD44
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Dentistry Publications
 
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Created: Mon, 02 Dec 2013, 13:21:32 EST by Roheen Gill on behalf of UQ Centre for Clinical Research