Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

Cerruti, Benedetta, Puliafito, Alberto, Shewan, Annette M., Yu, Wei, Combes, Alexander N., Little, Melissa H., Chianale, Federica, Primo, Luca, Serini, Guido, Mostov, Keith E., Celani, Antonio and Gamba, Andrea (2013) Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures. Journal of Cell Biology, 203 2: 359-372. doi:10.1083/jcb.201305044

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Author Cerruti, Benedetta
Puliafito, Alberto
Shewan, Annette M.
Yu, Wei
Combes, Alexander N.
Little, Melissa H.
Chianale, Federica
Primo, Luca
Serini, Guido
Mostov, Keith E.
Celani, Antonio
Gamba, Andrea
Title Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
Publication date 2013-10-28
Year available 2013
Sub-type Article (original research)
DOI 10.1083/jcb.201305044
Open Access Status File (Publisher version)
Volume 203
Issue 2
Start page 359
End page 372
Total pages 14
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Collection year 2014
Language eng
Abstract The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell–cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell–cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis.
Keyword Mitotic spindle orientation
Vascular lumen formation
Apical surface
Geometric order
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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