A comparison of hepatic ischemia/hypoxia-reperfusion injury models

Arab, Hossein-Ali, Cheung, Kee, Hickman, Peter E., Potter, Julia M., Walker, Neal I. and Roberts, Michael S. (2013) A comparison of hepatic ischemia/hypoxia-reperfusion injury models. Journal of Pharmacological and Toxicological Methods, 68 3: 349-356. doi:10.1016/j.vascn.2013.03.002

Author Arab, Hossein-Ali
Cheung, Kee
Hickman, Peter E.
Potter, Julia M.
Walker, Neal I.
Roberts, Michael S.
Title A comparison of hepatic ischemia/hypoxia-reperfusion injury models
Journal name Journal of Pharmacological and Toxicological Methods   Check publisher's open access policy
ISSN 1056-8719
Publication date 2013
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.vascn.2013.03.002
Open Access Status DOI
Volume 68
Issue 3
Start page 349
End page 356
Total pages 8
Place of publication Philadelphia, PA United States
Publisher Elsevier Inc.
Collection year 2014
Language eng
Subject 3004 Pharmacology
3005 Toxicology
Abstract Introduction: A number of hepatic ischemia/hypoxia-reperfusion models have been described. This study characterised the functional and structural changes induced by the most commonly used in vivo and in situ models for hypoxia/ischemia-reperfusion in the rat liver. Methods: A range of no-flow, slow-flow and lobar ischemia and reperfusion models were established in the rat liver. Changes following reperfusion were monitored using physiological, biochemical, histological and pharmacological assessments, including bile production, oxygen consumption, lignocaine extraction, enzyme release, and disposition of exogenous markers. Results: Short periods of hepatic ischemia/hypoxia-reperfusion led to minimal changes in liver function whereas long periods of ischemia-reperfusion led to substantial liver injury. The most severe injury was found with the slow flow, reflow model. The formation of cell vacuoles, blebs and focal hepatitis were the most important liver morphological changes observed as a consequence of ischemia/hypoxia. The major liver histological findings after reperfusion were dispersed apoptosis and local necrosis. Hepatic ischemia/hypoxia-reperfusion was also associated with significant changes in the hepatic extracellular and intracellular spaces. Discussion: The morphology and function of the liver associated with a range of hepatic ischemia/hypoxia-reperfusion models varies with the duration of the insult and between models. The choice of model is therefore an important consideration in seeking to resolve any particular hypothesis associated with hepatic ischemia/hypoxia-reperfusion.
Keyword Apoptosis
Liver function
Liver ischemia
Reperfusion injury
Slow flow
Solute disposition
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 28 Nov 2013, 21:02:40 EST by System User on behalf of Medicine - Princess Alexandra Hospital