The West Nile virus assembly process evades the conserved antiviral mechanism of the interferon-induced MxA protein

Hoenen, Antje, Gillespie, Leah, Morgan, Garry, van der Heide, Peter, Khromykh, Alexander and Mackenzie, Jason (2014) The West Nile virus assembly process evades the conserved antiviral mechanism of the interferon-induced MxA protein. Virology, 448 104-116. doi:10.1016/j.virol.2013.10.005


Author Hoenen, Antje
Gillespie, Leah
Morgan, Garry
van der Heide, Peter
Khromykh, Alexander
Mackenzie, Jason
Title The West Nile virus assembly process evades the conserved antiviral mechanism of the interferon-induced MxA protein
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
Publication date 2014-01-05
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.virol.2013.10.005
Volume 448
Start page 104
End page 116
Total pages 13
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2014
Language eng
Abstract Flaviviruses have evolved means to evade host innate immune responses. Recent evidence suggests this is due to prevention of interferon production and signaling in flavivirus-infected cells. Here we show that the interferon-induced MxA protein can sequester the West Nile virus strain Kunjin virus (WNVKUN) capsid protein in cytoplasmic tubular structures in an expression-replication system. This sequestering resulted in reduced titers of secreted WNVKUN particles. We show by electron microscopy, tomography and 3D modeling that these cytoplasmic tubular structures form organized bundles. Additionally we show that recombinant ER-targeted MxA can restrict production of infectious WNVKUN under conditions of virus infection. Our results indicate a co-ordinated and compartmentalized WNVKUN assembly process may prevent recognition of viral components by MxA, particularly the capsid protein. This recognition can be exploited if MxA is targeted to intracellular sites of WNVKUN assembly. This results in further understanding of the mechanisms of flavivirus evasion from the immune system.
Keyword Innate immunity
Interferon-induced antiviral protein
MxA
Virus assembly
West Nile virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 22 October 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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