Comparison of surface plasmon resonance, resonant waveguide grating biosensing and enzyme linked immunosorbent assay (ELISA) in the evaluation of a dengue virus immunoassay

Hu, Dongmei, Fry, Scott R., Huang, Johnny X., Ding, Xixia, Qiu, Liwen, Pan, Yuxian, Chen, Yue, Jin, Jing, McElnea, Catriona, Buechler, Joe, Che, Xiaoyan and Cooper, Matthew A. (2013) Comparison of surface plasmon resonance, resonant waveguide grating biosensing and enzyme linked immunosorbent assay (ELISA) in the evaluation of a dengue virus immunoassay. Biosensors, 3 3: 297-311. doi:10.3390/bios3030297


Author Hu, Dongmei
Fry, Scott R.
Huang, Johnny X.
Ding, Xixia
Qiu, Liwen
Pan, Yuxian
Chen, Yue
Jin, Jing
McElnea, Catriona
Buechler, Joe
Che, Xiaoyan
Cooper, Matthew A.
Title Comparison of surface plasmon resonance, resonant waveguide grating biosensing and enzyme linked immunosorbent assay (ELISA) in the evaluation of a dengue virus immunoassay
Journal name Biosensors   Check publisher's open access policy
ISSN 2079-6374
Publication date 2013
Year available 2013
Sub-type Article (original research)
DOI 10.3390/bios3030297
Open Access Status DOI
Volume 3
Issue 3
Start page 297
End page 311
Total pages 15
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2014
Language eng
Subject 1308 Clinical Biochemistry
Abstract Two label-free biosensor platforms, Resonance Waveguide Grating (RWG) and Surface Plasmon Resonance (SPR), were used to rank a large panel of anti-dengue virus NS1 antibodies. Dengue non-structural 1 (NS1) protein is an established serological marker for the early detection of dengue infection. A variety of commercial dengue NS1 antigen capture immunoassays are available in both ELISA and lateral flow format. However, there is a significant scope to improve both the sensitivity and the specificity of those tests. The interactions of antibody (Ab)-antigen (Ag) were profiled, with weak interactions (KD = 1-0.1 μM) able to be detected under static equilibrium conditions by RWG, but not observed to under more rigorous flow conditions using SPR. There were significant differences in the absolute affinities determined by the two technologies, and there was a poor correlation between antibodies best ranked by RWG and the lower limit of detection (LLOD) found by ELISA. Hence, whilst high-throughput RWG can be useful as preliminary screening for higher affinity antibodies, care should be exercised in the assignation of quantitative values for affinity between different assay formats.
Keyword Affinity
Dengue virus
Diagnostic
Kinetics
Label-free
Resonance waveguide grating
Surface plasmon resonance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 28 Nov 2013, 19:40:16 EST by System User on behalf of Institute for Molecular Bioscience