Proteomic analysis of glucohexaose induced resistance to downy mildew in Cucumis sativus

Hao, Yuhan, Wu, Chunfei, Zhao, Dawei, Thung, Leena, Yu, Yang and Fan, Haiyan (2013) Proteomic analysis of glucohexaose induced resistance to downy mildew in Cucumis sativus. Australian Journal of Crop Science, 7 9: 1242-1251.

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Author Hao, Yuhan
Wu, Chunfei
Zhao, Dawei
Thung, Leena
Yu, Yang
Fan, Haiyan
Title Proteomic analysis of glucohexaose induced resistance to downy mildew in Cucumis sativus
Formatted title
Proteomic analysis of glucohexaose induced resistance to downy mildew in Cucumis sativus
Journal name Australian Journal of Crop Science   Check publisher's open access policy
ISSN 1835-2693
1835-2707
Publication date 2013-08
Year available 2013
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 7
Issue 9
Start page 1242
End page 1251
Total pages 10
Place of publication Lismore, Australia
Publisher Southern Cross Journals
Collection year 2014
Formatted abstract
Glucohexaose, as one of synthetic oligosaccharides, induces the resistance response to protect plants from pathogen infection by inducing the systemic acquired resistance - like (SAR - like) response. To study the molecular mechanism of glucohexaose induced resistance, we investigate the physiological, biochemical and proteomic changes after glucohexaose treatment. The results shows cucumber plants had the highest protection level of 66.79% 48 h after the third times of 10 μg mL -1 glucohexaose treatment. Significant increases in chlorophyll, photosynthetic rate, soluble sugar,  leave dry weight and H2O2 were observed after glucohexaose treatment. Eighteen up - regulated proteins were identified by MALDI-TOF/TOF in glucohexaose-treated plants, predicted to be involved in photosynthesis, photorespiration, oxidative burst, transcriptional regulation, signal transduction and pathogen defense processes. The identification of up-regulated proteins involved in photosynthetic processes is a significant finding which suggests that a boost in metabolites is required for repartition of resources towards defense mechanisms. The proteins which responded to glucohexaose also included those associated with oxidative burst response, such as APX and isocitrate dehydrogenase. More comprehensive studies about the link between the molecular mechanisms regulated by ROS mediated photosynthesis and cucumber induced resistance by glucohexaose, are necessary in the future to broaden our understanding of induced resistance in plants.
Keyword 2-DE
Cucumber
Glucohexaose
Induced resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
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Created: Thu, 28 Nov 2013, 18:18:43 EST by System User on behalf of School of Agriculture and Food Sciences