Neonatal hypoxia-ischaemia disrupts descending neural inputs to dorsal raphé nuclei

Reinebrant H.E., Wixey J.A. and Buller K.M. (2013) Neonatal hypoxia-ischaemia disrupts descending neural inputs to dorsal raphé nuclei. Neuroscience, 248 427-435. doi:10.1016/j.neuroscience.2013.06.016


Author Reinebrant H.E.
Wixey J.A.
Buller K.M.
Title Neonatal hypoxia-ischaemia disrupts descending neural inputs to dorsal raphé nuclei
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
1873-7544
Publication date 2013-09-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.neuroscience.2013.06.016
Volume 248
Start page 427
End page 435
Total pages 9
Place of publication Oxford United Kingdom
Publisher Pergamon
Collection year 2014
Language eng
Formatted abstract
Neuronal losses have been shown to occur in the brainstem following a neonatal hypoxic-ischaemic (HI) insult. In particular serotonergic neurons, situated in the dorsal raphé nuclei, appear to be vulnerable to HI injury. Nonetheless the mechanisms contributing to losses of serotonergic neurons in the brainstem remain to be elucidated. One possible mechanism is that disruption of neural projections from damaged forebrain areas to dorsal raphé nuclei may play a role in the demise of serotonergic neurons. To test this, postnatal day 3 (P3) rat pups underwent unilateral common carotid artery ligation followed by hypoxia (6% O2 for 30min). On P38 a retrograde tracer, fluorescent-coupled choleratoxin b, was deposited in the dorsal raphé dorsal (DR dorsal) nucleus or the dorsal raphé ventral (DR ventral) nucleus. Compared to control animals, P3 HI animals had significant losses of retrogradely labelled neurons in the medial prefrontal cortex, preoptic area and lateral habenula after tracer deposit in the DR dorsal nucleus. On the other hand, after tracer deposit in the DR ventral nucleus, we found significant reductions in numbers of retrogradely labelled neurons in the hypothalamus, preoptic area and medial amygdala in P3 HI animals compared to controls. Since losses of descending inputs are associated with decreases in serotonergic neurons in the brainstem raphé nuclei, we propose that disruption of certain descending neural inputs from the forebrain to the DR dorsal and the DR ventral nuclei may contribute to losses of serotonergic neurons after P3 HI. It is important to delineate the phenotypes of different neuronal networks affected by neonatal HI, and the mechanisms underpinning this damage, so that interventions can be devised to target and protect axons from the harmful effects of neonatal HI.
Keyword Choleratoxin subunit b
Dorsal raphé nuclei
Hypoxia-ischaemia
Neonate
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 29 Nov 2013, 03:47:17 EST by System User on behalf of UQ Centre for Clinical Research