α-Fucosidases with different substrate specificities from two species of Fusarium

Paper, Janet M., Scott-Craig, John S., Cavalier, David, Faik, Ahmed, Wiemels, Richard E., Borrusch, Melissa S., Bongers, Mareike and Walton, Jonathan D. (2013) α-Fucosidases with different substrate specificities from two species of Fusarium. Applied Microbiology and Biotechnology, 97 12: 5371-5380. doi:10.1007/s00253-012-4423-3


Author Paper, Janet M.
Scott-Craig, John S.
Cavalier, David
Faik, Ahmed
Wiemels, Richard E.
Borrusch, Melissa S.
Bongers, Mareike
Walton, Jonathan D.
Title α-Fucosidases with different substrate specificities from two species of Fusarium
Formatted title
α-Fucosidases with different substrate specificities from two species of Fusarium 
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1007/s00253-012-4423-3
Volume 97
Issue 12
Start page 5371
End page 5380
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2014
Formatted abstract
Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 α-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.
Keyword Fucose
Fusarium graminearum
Fusarium oxysporum
Xyloglucan
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 29 Nov 2013, 02:27:48 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology