Single molecule FRET using the FRET pair DRONPA/PhotoActivable mCherry

Devauges, Viviane, Ortiz-Zapater, Elena, Efthymiou, Christina, Keppler, Melanie, Barbeau, Jody, Matthews, Daniel R., Monypenny of Pitmilly, James, Barber, Paul R., Rolfe, Daniel, Ng, Tony and Ameer-Beg, Simon M. (2013). Single molecule FRET using the FRET pair DRONPA/PhotoActivable mCherry. In: Single Molecule Spectroscopy and Superresolution Imaging VI. Single Molecule Spectroscopy and Superresolution Imaging VI, San Francisco, CA, (). 2 - 3 February 2013. doi:10.1117/12.2002470

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Author Devauges, Viviane
Ortiz-Zapater, Elena
Efthymiou, Christina
Keppler, Melanie
Barbeau, Jody
Matthews, Daniel R.
Monypenny of Pitmilly, James
Barber, Paul R.
Rolfe, Daniel
Ng, Tony
Ameer-Beg, Simon M.
Title of paper Single molecule FRET using the FRET pair DRONPA/PhotoActivable mCherry
Conference name Single Molecule Spectroscopy and Superresolution Imaging VI
Conference location San Francisco, CA
Conference dates 2 - 3 February 2013
Proceedings title Single Molecule Spectroscopy and Superresolution Imaging VI
Journal name Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Place of Publication Bellingham, WA United States
Publisher S P I E - International Society for Optical Engineering
Publication Year 2013
Year available 2013
Sub-type Fully published paper
DOI 10.1117/12.2002470
Open Access Status
ISBN 9780819493590
ISSN 1605-7422
Volume 8590
Total pages 9
Collection year 2014
Language eng
Abstract/Summary Photoswitchable and photoactivable proteins Dronpa and PhotoActivable mCherry (PA-mCherry) respectively, were used in order to perform FRET (Förster Resonance Energy Transfer) imaging at the single molecule level, using a FRET standard construct consisting of Dronpa and PA-mCherry separated by seven amino acids expressed in cells. Given Dronpa's complex photophysical properties and the existence of a preswitched emissive state, irradiation conditions at 491 and 405 nm were optimised. We discuss strategies for observing FRET at the single molecule level with photoactivatable proteins by monitoring modifications in the donor and acceptors emissive states.
Subjects 3107 Atomic and Molecular Physics, and Optics
2504 Electronic, Optical and Magnetic Materials
2502 Biomaterials
2741 Radiology Nuclear Medicine and imaging
Keyword Photoswitchable/photoactivable proteins
Single Molecule Imaging Microscopy
Forster Resonance Energy Transfer (FRET)
Fluorescent Lifetime Imaging Microscopy (FLIM)
Q-Index Code E1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Conference Paper
Collections: Non HERDC
Queensland Brain Institute Publications
 
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