Same, same but different: symbiotic bacterial associations in GBR sponges

Webster, N. S., Luter, H. M., Soo, R. M., Botté, E. S., Simister, R. L., Abdo, D. and Whalan, S. (2012) Same, same but different: symbiotic bacterial associations in GBR sponges. Frontiers in Microbiology, 3 444: 1-11. doi:10.3389/fmicb.2012.00444


Author Webster, N. S.
Luter, H. M.
Soo, R. M.
Botté, E. S.
Simister, R. L.
Abdo, D.
Whalan, S.
Title Same, same but different: symbiotic bacterial associations in GBR sponges
Journal name Frontiers in Microbiology   Check publisher's open access policy
ISSN 1664-302X
Publication date 2012-01-18
Sub-type Article (original research)
DOI 10.3389/fmicb.2012.00444
Open Access Status DOI
Volume 3
Issue 444
Start page 1
End page 11
Total pages 11
Editor Karla B. Heidelberg
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Subject 2404 Microbiology
2726 Microbiology (medical)
Formatted abstract
Symbioses in marine sponges involve diverse consortia of microorganisms that contribute to the health and ecology of their hosts. The microbial communities of 13 taxonomically diverse Great Barrier Reef (GBR) sponge species were assessed by DGGE and 16S rRNA gene sequencing to determine intra and inter species variation in bacterial symbiont composition. Microbial profiling revealed communities that were largely conserved within different individuals of each species with intra species similarity ranging from 65-100%. 16S rRNA gene sequencing revealed that the communities were dominated by Proteobacteria, Chloroflexi, Acidobacteria, Actinobacteria, Nitrospira,and Cyanobacteria. Sponge-associated microbes were also highly host-specific with no operational taxonomic units (OTUs) common to all species and the most ubiquitous OTU found in only 5 of the 13 sponge species. In total, 91% of the OTUs were restricted to a single sponge species. However, GBR sponge microbes were more closely related to other sponge-derived bacteria than they were to environmental communities with sequences falling within 50 of the 173 previously defined sponge-(or sponge-coral) specific sequence clusters (SC). These SC spanned the Acidobacteria, Actinobacteria, Proteobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Gemmatimonadetes, Nitrospira,and the Planctomycetes-Verrucomicrobia-Chlamydiae superphylum. The number of sequences assigned to these sponge-specific clusters across all species ranged from 0 to 92%. No relationship between host phylogeny and symbiont communities were observed across the different sponge orders, although the highest level of similarity was detected in two closely related Xestospongia species. This study identifies the core microbial inhabitants in a range of GBR sponges thereby providing the basis for future studies on sponge symbiotic function and research aiming to predict how sponge holobionts will respond to environmental perturbation.
Keyword Sponge
Microorganism
Symbiont
Diversity
Great Barrier Reef
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Centre for Ecogenomics
 
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