The FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells

Amelung, Silva, Nerlich, Andreas, Rohde, Manfred, Spellerberg, Barbara, Cole, Jason N., Nizet, Victor, Chhatwal, Gursharan S. and Talay, Susanne R. (2011) The FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells. Cellular Microbiology, 13 8: 1200-1211. doi:10.1111/j.1462-5822.2011.01610.x


Author Amelung, Silva
Nerlich, Andreas
Rohde, Manfred
Spellerberg, Barbara
Cole, Jason N.
Nizet, Victor
Chhatwal, Gursharan S.
Talay, Susanne R.
Title The FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells
Formatted title
The FbaB-type fibronectin-binding protein of Streptococcus pyogenes promotes specific invasion into endothelial cells
Journal name Cellular Microbiology   Check publisher's open access policy
ISSN 1462-5814
1462-5822
Publication date 2011-08
Sub-type Article (original research)
DOI 10.1111/j.1462-5822.2011.01610.x
Open Access Status
Volume 13
Issue 8
Start page 1200
End page 1211
Total pages 12
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 2404 Microbiology
2403 Immunology
2406 Virology
Formatted abstract
Invasive serotype M3 Streptococcus pyogenes are among the most frequently isolated organisms from patients suffering from invasive streptococcal disease and have the potential to invade primary human endothelial cells (EC) via a rapid and efficient mechanism. FbaB protein, the fibronectin-binding protein expressed by M3 S. pyogenes, was herein identified as a potent invasin for EC. By combining heterologous gene expression with allelic replacement, we demonstrate that FbaB is essential and sufficient to trigger EC invasion via a Rac1-dependent phagocytosis-like uptake. FbaB-mediated uptake follows the classical endocytic pathway with lysosomal destination. FbaB is demonstrated to be a streptococcal invasin exhibiting EC tropism. FbaB thus initiates a process that may contribute to the deep tissue tropism and spread of invasive S. pyogenes isolates into the vascular EC lining.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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