Confocal Raman microspectroscopic study of the molecular status of carotenoids in tomato fruits and foods

Pudney, Paul, Gambelli, Luisa and Gidley, Michael J. (2011) Confocal Raman microspectroscopic study of the molecular status of carotenoids in tomato fruits and foods. Applied Spectroscopy, 65 2: 127-134. doi:10.1366/10-06121


Author Pudney, Paul
Gambelli, Luisa
Gidley, Michael J.
Title Confocal Raman microspectroscopic study of the molecular status of carotenoids in tomato fruits and foods
Journal name Applied Spectroscopy   Check publisher's open access policy
ISSN 0003-7028
1943-3530
Publication date 2011-02
Sub-type Article (original research)
DOI 10.1366/10-06121
Open Access Status
Volume 65
Issue 2
Start page 127
End page 134
Total pages 8
Place of publication Frederick, MD, United States
Publisher Society for Applied Spectroscopy
Language eng
Abstract To understand the uptake into the body of bioactive "healthy" molecules from fruits and vegetables it is necessary to know where they are located within the original plant structures and what path they take as the plant is processed before consumption. This means not only the location but also the physical state of the molecule, e.g., crystalline or solvated, as this affects uptake. This study demonstrates how confocal Raman microspectroscopy can be used to follow changes in the physical state of carotenoids in tomatoes. We show how the three main carotenoids found in tomatoes, Iycopene, β-carotene, and lutein, are distinguished from each other and how their physical state can be determined. We then show how this information can be used in situ within tomato fruit organelles, in three different tomato varieties, to locate the different carotenoids and determine their physical status. The effect of processing on carotenoids in tomatoes is also shown. This type of information can be of great benefit in availability trials to help rationalize results and define the state in which foods should be presented to the body to maximize uptake.
Keyword β-Carotene
Food products
Lutein
Lycopene
MCR
Multivariate curve resolution
Plants
Raman mapping
Raman spectroscopy
Tomato
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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