c-Myc regulation and apoptosis in assessing the beneficial effect of apigenin in cyclosporine induced nephrotoxicity

Chakravarthi, Srikumar, Haleagrahara, Nagaraja, Wen, Chong Fu, Lee, Nagaraja and Thani, P. M. (2010) c-Myc regulation and apoptosis in assessing the beneficial effect of apigenin in cyclosporine induced nephrotoxicity. Research Journal of Pharmacology, 4 1: 15-20. doi:10.3923/rjpharm.2010.15.20


Author Chakravarthi, Srikumar
Haleagrahara, Nagaraja
Wen, Chong Fu
Lee, Nagaraja
Thani, P. M.
Title c-Myc regulation and apoptosis in assessing the beneficial effect of apigenin in cyclosporine induced nephrotoxicity
Journal name Research Journal of Pharmacology
ISSN 1815-9362
1993-6109
Publication date 2010
Sub-type Article (original research)
DOI 10.3923/rjpharm.2010.15.20
Open Access Status
Volume 4
Issue 1
Start page 15
End page 20
Total pages 6
Place of publication Faisalabad, Pakistan
Publisher Medwell Journals
Language eng
Subject 2736 Pharmacology (medical)
3000 Pharmacology, Toxicology and Pharmaceutics
Formatted abstract
Cyclosporine-A (CsA) is an immunosuppressant prescribed in organ transplants to prevent rejection. It is a calcineurin inhibitor produced by the fungi Trichoderma polysporum and Cylindrocarpon lucidum, its adverse effect of renal dysfunction has limited its use in a clinical setting. Apigenin (4′, 5′,7′-Trihydroxyflavone), a herbal extract, with anti-inflammatory and anti-tumour properties has shown to reverse this adverse effect. This research was conducted to study the effects of apigenin on reversal of Cyclosporine-A induced damage and this was assessed by immunohistochemical estimation of expression of c-myc and estimation of apoptosis in histopathological sections. Rats were divided into groups and administered with CsA with Apigenin in different doses. The kidneys from the rats were harvested, weighed and observed for gross pathology changes. The renal tissue was processed and stained for haemotoxylin and eosin staining, to assess the apoptotic index and stained by immunohistochemistry, for the analysis of the apoptosis regulatory gene c-myc. The apoptotic index was then compared with the c-myc intensity to observe for any correlation. It was found that there was a high apoptotic index and c-myc intensity in the Cyclosporine-A group. Apigenin managed to reduce the values of both parameters. The apoptotic index correlated with the c-myc intensity, especially in the glomeruli. The study proved that Cyclosporine-A enhanced the expression of c-myc in the rat kidney, which signifies accelerated apoptosis. Therefore, c-myc and apoptotic index may be used to assess apigenin and its effect on Cyclosporine-A induced renal damage.
Keyword Cyclosporine
Nephrotoxicity
Apigenin
4′,5′,7′- Trihydroxyflavone
Apoptosis
c-Myc
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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