Highly efficient, 5′-sequence-specific transgene silencing in a complex polyploid

Birch, Robert G., Bower, Robert S. and Elliott, Adrian R. (2010) Highly efficient, 5′-sequence-specific transgene silencing in a complex polyploid. Tropical Plant Biology, 3 2: 88-97. doi:10.1007/s12042-010-9047-0


Author Birch, Robert G.
Bower, Robert S.
Elliott, Adrian R.
Title Highly efficient, 5′-sequence-specific transgene silencing in a complex polyploid
Journal name Tropical Plant Biology   Check publisher's open access policy
ISSN 1935-9756
1935-9764
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1007/s12042-010-9047-0
Open Access Status
Volume 3
Issue 2
Start page 88
End page 97
Total pages 10
Place of publication New York, United States
Publisher Springer
Language eng
Abstract Sugarcane, a complex polyploid, shows highly efficient and rapidly imposed silencing of diverse transgene constructs. Silencing is 5′-sequence-specific, copy-number independent, developmentally regulated and post-transcriptional in the plants first regenerated from transgenic callus. The results challenge some early generalizations from studies in model dicotyledonous plants, about causes of transgene silencing and approaches to avoid the problem. We reason that patchy and progressive transgene silencing, seen in most other plant species, may be associated with increasing endopolyploidy during maturation of differentiated tissues. The experimentally tractable sugarcane system allows features likely to trigger or protect from silencing to be tested, and ultimately controlled to improve the efficiency of plant improvement by genetic transformation.
Keyword Post-transcriptional silencing
Ubiquitin promoter
Luciferase
Sugarcane
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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