Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis

Picardeau, Mathieu P., Bulach, Dieter M., Bouchier, Christiane, Zuerner, Richard L., Zidane, Nora, Wilson, Peter J., Creno, Sophie, Kuczek, Elizabeth S., Bommezzadri, Simona, Davis, John C., McGrath, Annette, Johnson, Matthew J., Boursaux-Eude, Caroline, Seemann, Torsten, Rouy, Zoe, Coppel, Ross L., Rood, Julian I., Lajus, Aurelie, Davies, John K., Medigue, Claudine and Adler, Ben (2008) Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS One, 3 2: . doi:10.1371/journal.pone.0001607


Author Picardeau, Mathieu P.
Bulach, Dieter M.
Bouchier, Christiane
Zuerner, Richard L.
Zidane, Nora
Wilson, Peter J.
Creno, Sophie
Kuczek, Elizabeth S.
Bommezzadri, Simona
Davis, John C.
McGrath, Annette
Johnson, Matthew J.
Boursaux-Eude, Caroline
Seemann, Torsten
Rouy, Zoe
Coppel, Ross L.
Rood, Julian I.
Lajus, Aurelie
Davies, John K.
Medigue, Claudine
Adler, Ben
Title Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis
Formatted title
Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis 
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-02-13
Sub-type Article (original research)
DOI 10.1371/journal.pone.0001607
Open Access Status DOI
Volume 3
Issue 2
Total pages 9
Place of publication San Francisco, United States
Publisher Public Library of Science
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Formatted abstract
Leptospira biflexa is a free-living saprophytic spirochete present in aquatic environments. We determined the genome sequence of L. biflexa, making it the first saprophytic Leptospira to be sequenced. The L. biflexa genome has 3,590 protein-coding genes distributed across three circular replicons: the major 3,604 chromosome, a smaller 278-kb replicon that also carries essential genes, and a third 74-kb replicon. Comparative sequence analysis provides evidence that L. biflexa is an excellent model for the study of Leptospira evolution; we conclude that 2052 genes (61%) represent a progenitor genome that existed before divergence of pathogenic and saprophytic Leptospira species. Comparisons of the L. biflexa genome with two pathogenic Leptospira species reveal several major findings. Nearly one-third of the L. biflexa genes are absent in pathogenic Leptospira. We suggest that once incorporated into the L. biflexa genome, laterally transferred DNA undergoes minimal rearrangement due to physical restrictions imposed by high gene density and limited presence of transposable elements. In contrast, the genomes of pathogenic Leptospira species undergo frequent rearrangements, often involving recombination between insertion sequences. Identification of genes common to the two pathogenic species, L. borgpetersenii and L. interrogans, but absent in L. biflexa, is consistent with a role for these genes in pathogenesis. Differences in environmental sensing capacities of L. biflexa, L. borgpetersenii, and L. interrogans suggest a model which postulates that loss of signal transduction functions in L. borgpetersenii has impaired its survival outside a mammalian host, whereas L. interrogans has retained environmental sensory functions that facilitate disease transmission through water. 
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Science Publications
 
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