A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family

Hansen, Martin A., Nielsen, John E., Retelska, Dorota, Larsen, Niels and Leffers, Hendrik (2008) A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family. Molecular Reproduction and Development, 75 2: 219-229. doi:10.1002/mrd.20651


Author Hansen, Martin A.
Nielsen, John E.
Retelska, Dorota
Larsen, Niels
Leffers, Hendrik
Title A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family
Journal name Molecular Reproduction and Development   Check publisher's open access policy
ISSN 1040-452X
1098-2795
Publication date 2008
Year available 2008
Sub-type Article (original research)
DOI 10.1002/mrd.20651
Open Access Status
Volume 75
Issue 2
Start page 219
End page 229
Total pages 11
Place of publication Hoboken, NJ United States
Publisher John Wiley and Sons, Inc.
Collection year 2009
Language eng
Subject 1311 Genetics
1309 Developmental Biology
1307 Cell Biology
Abstract Many testis-specific genes from the sex chromosomes are subject to rapid evolution, which can make it difficult to identify murine genes in the human genome. The murine CYPT gene family includes 15 members, but orthologs were undetectable in the human genome. However, using refined homology search, sequences corresponding to the shared promoter region of the CYPT family were identified at 39 loci. Most loci were located immediately upstream of genes belonging to the VCX/Y, SPANX, or CSAG gene families. Sequence comparison of the loci revealed a conserved CYPT promoter-like (CPL) element featuring TATA and CCAAT boxes. The expression of members of the three families harboring the CPL resembled the murine expression of the CYPT family, with weak expression in late pachytene spermatocytes and predominant expression in spermatids, but some genes were also weakly expressed in somatic cells and in other germ cell types. The genomic regions harboring the gene families were rich in direct and inverted segmental duplications (SD), which may facilitate gene conversion and rapid evolution. The conserved CPL and the common expression profiles suggest that the human VCX/Y, SPANX, and CSAG2 gene families together with the murine SPANX gene and the CYPT family may share a common ancestor. Finally, we present evidence that VCX/Y and SPANX may be paralogs with a similar protein structure consisting of C terminal acidic repeats of variable lengths.
Keyword Evolution
Gene expression
Promoter
Spermatogenesis
Testis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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