Transcriptional regulatory networks in macrophages

Hume D.A., Wells C.A. and Ravasi T. (2007). Transcriptional regulatory networks in macrophages. In: , , (2-18). .

Author Hume D.A.
Wells C.A.
Ravasi T.
Title of paper Transcriptional regulatory networks in macrophages
Journal name Novartis Foundation Symposium   Check publisher's open access policy
Publication Year 2007
Sub-type Fully published paper
ISBN 9780470027554
ISSN 1528-2511
Volume 281
Start page 2
End page 18
Total pages 17
Abstract/Summary The functions of macrophages in the innate immune system require the constitutive expression of a wide range of myeloid-specific genes, including various pattern recognition receptors, as well as the inducible expression of a suite of genes required to initiate inflammation and eliminate pathogens. Our overall aim is to understand the transcriptional networks that underlie both macrophage-specific transcription and the response to pathogen components such as lipopolysaccharide (LPS). The approaches used include detailed functional analysis of specific promoters, such as that of the CSF1 receptor, global cDNA microarray expression profiling, high throughput real-time PCR analysis of all the transcription factors encoded by the mammalian genome, full length cDNA library construction and sequencing, CAGE analysis to identify specific promoters used in macrophages and motif analysis to detect candidate cis-acting elements in co-regulated genes in macrophages. This review discusses some of the progress in moving towards a transcriptional network model for mouse macrophage activation by LPS, as well as insight into the role of alternative promoter usage and polyadenylation in generating functional protein variants that impact on signalling in macrophages. Copyright
Subjects 2700 Medicine
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: Scopus Import
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Created: Tue, 26 Nov 2013, 21:16:47 EST by System User