A role for ARF6 and ARNO in the regulation of endosomal dynamics in neurons

Hernandez-Deviez D., Mackay-Sim A. and Wilson J.M. (2007) A role for ARF6 and ARNO in the regulation of endosomal dynamics in neurons. Traffic, 8 12: 1750-1764. doi:10.1111/j.1600-0854.2007.00649.x


Author Hernandez-Deviez D.
Mackay-Sim A.
Wilson J.M.
Title A role for ARF6 and ARNO in the regulation of endosomal dynamics in neurons
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
Publication date 2007
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2007.00649.x
Volume 8
Issue 12
Start page 1750
End page 1764
Total pages 15
Subject 1303 Specialist Studies in Education
1307 Cell Biology
1315 Structural Biology
1312 Molecular Biology
1311 Genetics
Abstract During development, neuronal processes extend, branch and navigate to ultimately synapse with target tissue. We have shown a regulatory role for ARNO and ARF6 in dendritic branching and axonal elongation and branching during neuritogenesis, particularly with respect to cytoskeletal dynamics. Here, we have examined the role of ARF6 and the ARF GEF ARNO in endosomal dynamics during neurite elongation in hippocampal neurons. Axonal and dendritic endosomes were labeled by expression of the endosomal marker, endotubin. Expression of endotubin-green fluorescent protein resulted in targeting to tubular-vesicular structures throughout the somatodendritic and axonal domains. These endosomal structures did not colocalize with conventional early or late endosomal markers or with the synaptic vesicle marker, SV2. However, they did label with internalized lectin, indicating that they are endosomal structures. Expression of catalytically inactive ARNO (ARNO-E156K) or inactive ARF6 (ARF6-T27N) caused a redistribution of endotubin to the cell surface of the axons and dendrites. In contrast, expression of these constructs had no effect upon the distribution of SV2-positive structures. Furthermore, expression of inactive ARF1 (ARF1-T31N) did not change endotubin distribution. These results suggest that endotubin labels a distinct endosomal structure in neurons and that ARNO and ARF6 mediate neurite extension through the regulation of this compartment. © 2007 The Authors Journal compilation
Keyword ARF6
ARNO
Endosomes
Membrane traffic
Neurons
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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