PTRF/cavin-1 neutralizes non-caveolar caveolin-1 microdomains in prostate cancer

Moon, H., Lee, C. S., Inder, K. L., Sharma, S., Choi, E., Black, D. M., Lê Cao, K.-A., Winterford, C., Coward, J. I., Ling, M. T., Craik, D. J., Parton, R. G., Russell, P.J., Hill, M. M. and The Australian Prostate Cancer BioResource (2014) PTRF/cavin-1 neutralizes non-caveolar caveolin-1 microdomains in prostate cancer. Oncogene, 33 27: 3561-3570. doi:10.1038/onc.2013.315

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Author Moon, H.
Lee, C. S.
Inder, K. L.
Sharma, S.
Choi, E.
Black, D. M.
Lê Cao, K.-A.
Winterford, C.
Coward, J. I.
Ling, M. T.
Craik, D. J.
Parton, R. G.
Russell, P.J.
Hill, M. M.
The Australian Prostate Cancer BioResource
Total Author Count Override 15
Title PTRF/cavin-1 neutralizes non-caveolar caveolin-1 microdomains in prostate cancer
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
1476-5594
Publication date 2014
Year available 2013
Sub-type Article (original research)
DOI 10.1038/onc.2013.315
Open Access Status
Volume 33
Issue 27
Start page 3561
End page 3570
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2014
Language eng
Formatted abstract
Caveolin-1 has a complex role in prostate cancer and has been suggested to be a potential biomarker and therapeutic target. As mature caveolin-1 resides in caveolae, invaginated lipid raft domains at the plasma membrane, caveolae have been suggested as a tumor-promoting signaling platform in prostate cancer. However, caveola formation requires both caveolin-1 and cavin-1 (also known as PTRF; polymerase I and transcript release factor). Here, we examined the expression of cavin-1 in prostate epithelia and stroma using tissue microarray including normal, non-malignant and malignant prostate tissues. We found that caveolin-1 was induced without the presence of cavin-1 in advanced prostate carcinoma, an expression pattern mirrored in the PC-3 cell line. In contrast, normal prostate epithelia expressed neither caveolin-1 nor cavin-1, while prostate stroma highly expressed both caveolin-1 and cavin-1. Utilizing PC-3 cells as a suitable model for caveolin-1-positive advanced prostate cancer, we found that cavin-1 expression in PC-3 cells inhibits anchorage-independent growth, and reduces in vivo tumor growth and metastasis in an orthotopic prostate cancer xenograft mouse model. The expression of α-smooth muscle actin in stroma along with interleukin-6 (IL-6) in cancer cells was also decreased in tumors of mice bearing PC-3-cavin-1 tumor cells. To determine whether cavin-1 acts by neutralizing caveolin-1, we expressed cavin-1 in caveolin-1-negative prostate cancer LNCaP and 22Rv1 cells. Caveolin-1 but not cavin-1 expression increased anchorage-independent growth in LNCaP and 22Rv1 cells. Cavin-1 co-expression reversed caveolin-1 effects in caveolin-1-positive LNCaP cells. Taken together, these results suggest that caveolin-1 in advanced prostate cancer is present outside of caveolae, because of the lack of cavin-1 expression. Cavin-1 expression attenuates the effects of non-caveolar caveolin-1 microdomains partly via reduced IL-6 microenvironmental function. With circulating caveolin-1 as a potential biomarker for advanced prostate cancer, identification of the molecular pathways affected by cavin-1 could provide novel therapeutic targets.
Keyword PTRF
Cavin-1
Caveolin-1
Caveolae
Prostate cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Advance online publication 12 August 2013

 
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Created: Fri, 22 Nov 2013, 11:59:50 EST by Susan Allen on behalf of UQ Diamantina Institute