Mining the genome of Arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance

De Coninck, Barbara, Carron, Delphine, Tavormina, Patrizia, Willem, Lander, Craik, David J., Vos, Christine, Thevissen, Karin, Mathys, Janick and Cammue, Bruno P. A. (2013) Mining the genome of Arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance. Journal of Experimental Biology, 64 17: 5297-5307. doi:10.1093/jxb/ert295

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Author De Coninck, Barbara
Carron, Delphine
Tavormina, Patrizia
Willem, Lander
Craik, David J.
Vos, Christine
Thevissen, Karin
Mathys, Janick
Cammue, Bruno P. A.
Title Mining the genome of Arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance
Formatted title
Mining the genome of Arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance
Journal name Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
1477-9145
Publication date 2013-12
Sub-type Article (original research)
DOI 10.1093/jxb/ert295
Open Access Status File (Publisher version)
Volume 64
Issue 17
Start page 5297
End page 5307
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2014
Language eng
Formatted abstract
Although evidence has accumulated on the role of plant peptides in the response to external conditions, the number of peptide-encoding genes in the genome is still underestimated. Using tiling arrays, we identified 176 unannotated transcriptionally active regions (TARs) in Arabidopsis thaliana that were induced upon oxidative stress generated by the herbicide paraquat (PQ). These 176 TARs could be translated into 575 putative oxidative stress-induced peptides (OSIPs). A high-throughput functional assay was used in the eukaryotic model organism Saccharomyces cerevisiae allowing us to test for bioactive peptides that increase oxidative stress tolerance. In this way, we identified three OSIPs that, upon overexpression in yeast, resulted in a significant rise in tolerance to hydrogen peroxide (H2O2). For one of these peptides, the decapeptide OSIP108, exogenous application to H2O2-treated yeast also resulted in significantly increased survival. OSIP108 is contained within a pseudogene and is induced in A. thaliana leaves by both the reactive oxygen species-inducer PQ and the necrotrophic fungal pathogen Botrytis cinerea. Moreover, infiltration and overexpression of OSIP108 in A. thaliana leaves resulted in increased tolerance to treatment with PQ. In conclusion, the identification and characterization of OSIP108 confirms the validity of our high-throughput approach, based on tiling array analysis in A. thaliana and functional screening in yeast, to identify bioactive peptides.
Keyword Arabidopsis thaliana
Bioactive peptide
Hydrogen peroxide
Paraquat
Saccharomyces cerevisiae
sORF
Tiling array
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 22 Nov 2013, 11:07:59 EST by Susan Allen on behalf of Institute for Molecular Bioscience