In vivo evolution of antimicrobial resistance in a series of Staphylococcus aureus patient isolates: the entire picture or a cautionary tale?

van Hal, Sebastiaan J., Steen, Jason A., Espedido, Bjorn A., Grimmond, Sean M., Cooper, Matthew A., Holden, Matthew T. G., Bentley, Stephen D., Gosbell, Iain B. and Jensen, Slade O. (2014) In vivo evolution of antimicrobial resistance in a series of Staphylococcus aureus patient isolates: the entire picture or a cautionary tale?. Journal of Antimicrobial Chemotherapy, 69 2: 363-367. doi:10.1093/jac/dkt354


Author van Hal, Sebastiaan J.
Steen, Jason A.
Espedido, Bjorn A.
Grimmond, Sean M.
Cooper, Matthew A.
Holden, Matthew T. G.
Bentley, Stephen D.
Gosbell, Iain B.
Jensen, Slade O.
Title In vivo evolution of antimicrobial resistance in a series of Staphylococcus aureus patient isolates: the entire picture or a cautionary tale?
Formatted title
In vivo evolution of antimicrobial resistance in a series of Staphylococcus aureus patient isolates: the entire picture or a cautionary tale?
Journal name Journal of Antimicrobial Chemotherapy   Check publisher's open access policy
ISSN 0305-7453
1460-2091
Publication date 2014-02
Year available 2013
Sub-type Article (original research)
DOI 10.1093/jac/dkt354
Open Access Status
Volume 69
Issue 2
Start page 363
End page 367
Total pages 5
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2014
Language eng
Formatted abstract
Objectives: To obtain an expanded understanding of antibiotic resistance evolution in vivo, particularly in the context of vancomycin exposure.

Methods: The whole genomes of six consecutive methicillin-resistant Staphylococcus aureus blood culture isolates (ST239-MRSA-III) from a single patient exposed to various antimicrobials (over a 77 day period) were sequenced and analysed.

Results: Variant analysis revealed the existence of non-susceptible sub-populations derived from a common susceptible ancestor, with the predominant circulating clone(s) selected for by type and duration of antimicrobial exposure.

Conclusions: This study highlights the dynamic nature of bacterial evolution and that non-susceptible sub-populations can emerge from clouds of variation upon antimicrobial exposure. Diagnostically, this has direct implications for sample selection when using whole-genome sequencing as a tool to guide clinical therapy. In the context of bacteraemia, deep sequencing of bacterial DNA directly from patient blood samples would avoid culture ‘bias’ and identify mutations associated with circulating non-susceptible sub-populations, some of which may confer cross-resistance to alternate therapies.
Keyword MRSA
Sub-populations
Genomics
Diagnostics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 21 Nov 2013, 11:53:28 EST by Susan Allen on behalf of Institute for Molecular Bioscience