Host immune response determines visceral hyperalgesia in a rat model of post-inflammatory irritable bowel syndrome

Adam, Birgit, Tsopelas, Chris, Liebregts, Tobias, Bartholomeusz, F. Dylan and Holtmann, Gerald (2013) Host immune response determines visceral hyperalgesia in a rat model of post-inflammatory irritable bowel syndrome. Journal of Gastroenterology, 48 10: 1119-1127. doi:10.1007/s00535-012-0729-2


Author Adam, Birgit
Tsopelas, Chris
Liebregts, Tobias
Bartholomeusz, F. Dylan
Holtmann, Gerald
Title Host immune response determines visceral hyperalgesia in a rat model of post-inflammatory irritable bowel syndrome
Journal name Journal of Gastroenterology   Check publisher's open access policy
ISSN 0944-1174
1435-5922
Publication date 2013-10
Sub-type Article (original research)
DOI 10.1007/s00535-012-0729-2
Volume 48
Issue 10
Start page 1119
End page 1127
Total pages 9
Place of publication Tokyo, Japan
Publisher Springer
Collection year 2014
Language eng
Formatted abstract
Background: Irritable bowel syndrome (IBS) is associated with visceral hyperalgesia and frequently occurs after a transient gastrointestinal infection. Only a proportion of patients with acute gastroenteritis develop post-infectious IBS suggesting differences in host response to inflammatory stimuli. We aimed to investigate this concept by characterizing visceral sensitivity in two rat strains, following a chemically induced colitis.

Methods: Colorectal instillation of trinitrobenzenesulfonic acid (TNBS) in aqueous ethanol was used to induce a transient colitis in Lewis and F344 rats. The colitis was characterized semiquantitatively by histology, as well as by quantitative methods using 99mTc-leukocytes (radioactive organ assay) and plasma IL-2 and IL-6 levels. Visceromotor response to colorectal distensions was assessed after 2 h and, 5, 14, and 28 days.

Results: The colitis peaked on day 5 and dissipated to no visible mucosal damage on day 14. Cytokines were significantly increased in TNBS-treated rats at 2 h and on day 5. On day 14 cytokines were still significantly enhanced in Lewis but not Fisher rats. Both strains had a highly inflamed to non-inflamed tissue ratio at 3 h after TNBS instillation with increased uptake in Lewis compared to F344 rats. No 99mTc-tin-colloid-leukocytes were detected in colon samples on day 28. Visceromotor response was significantly elevated in both strains during the acute colitis (day 5), whereas only Lewis rats developed a post-inflammatory (day 28) visceral hyperalgesia.

Conclusion: Genetically determined host factors account for prolonged immune activation in response to a standardized inflammatory stimulus and are linked to susceptibility for a post-inflammatory visceral hyperalgesia.
Keyword Inflammation
Visceral hyperalgesia
Cytokines
Irritable bowel syndrome
Genetic factors
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
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