Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

Fuchs, Adrian V., Ritz, Sandra, Pütz, Sabine, Mailänder, Volker, Landfester, Katharina and Ziener, Ulrich (2013) Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties. Biomaterials Science, 1 5: 470-477. doi:10.1039/c2bm00155a


Author Fuchs, Adrian V.
Ritz, Sandra
Pütz, Sabine
Mailänder, Volker
Landfester, Katharina
Ziener, Ulrich
Title Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties
Journal name Biomaterials Science   Check publisher's open access policy
ISSN 2047-4830
2047-4849
Publication date 2013-05-01
Sub-type Article (original research)
DOI 10.1039/c2bm00155a
Open Access Status Not Open Access
Volume 1
Issue 5
Start page 470
End page 477
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher R S C Publications
Collection year 2014
Language eng
Subject 2500 Materials Science
2204 Religion and Religious Studies
Formatted abstract
The antibacterial (bioactive) and antifouling (biopassive) properties of stable, uniform, high surface coverage films of poly(hydroxyethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p(HEMA-co-MPC)) with embedded, non-leaching silver nanoparticles (AgNPs) are reported. Based on the experimental findings, a mechanism of action of AgNPs in antibacterial activity in combination with antifouling characteristics is discussed. Long-term antifouling studies of E. coli determine little to no adhesion on p(HEMA-co-MPC)/Ag films at 2.5 × 106 CFU mL−1 for 7 d, measured using live/dead staining assays. Agar diffusion tests indicate that there is no leaching of Ag from the films and SEM and EDX analyses of the films before and after incubation with E. coli show no attachment of E. coli and no visible change in film morphology or AgNP dispersal. Antibacterial studies are investigated using E. coli K-12 as a model bacterial strain and are tested in static (CFUs) and dynamic contact assays. Antibacterial efficacy of the films containing extremely low AgNP concentration (3.8 ng cm−2) is shown with growth suppression of E. coli in culture medium for 4 h at 1.35 × 105 CFU mL−1 and killing greater than 99% of E. coli in only 1 h of exposure to concentrations up to 1 × 105 CFU mL−1. These hybrid films may propose an exciting direction to long-term antibacterial and antifouling films in clinical applications.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 13 Nov 2013, 21:38:13 EST by Adrian Fuchs on behalf of Aust Institute for Bioengineering & Nanotechnology