Pasteurella multocida Heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity

Harper, Marina, St. Michael, Frank, John, Marietta, Vinogradov, Evgeny, Steen, Jennifer A., van Dorsten, Lieke, Steen, Jason A., Turni, Conny, Blackall, Patrick J., Adler, Ben, Cox, Andrew D. and Boyce, John D. (2013) Pasteurella multocida Heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity. Journal of Bacteriology, 195 21: 4854-4864. doi:10.1128/JB.00779-13

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Author Harper, Marina
St. Michael, Frank
John, Marietta
Vinogradov, Evgeny
Steen, Jennifer A.
van Dorsten, Lieke
Steen, Jason A.
Turni, Conny
Blackall, Patrick J.
Adler, Ben
Cox, Andrew D.
Boyce, John D.
Title Pasteurella multocida Heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity
Formatted title
Pasteurella multocida Heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
1098-5530
Publication date 2013-11
Sub-type Article (original research)
DOI 10.1128/JB.00779-13
Open Access Status File (Publisher version)
Volume 195
Issue 21
Start page 4854
End page 4864
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2014
Language eng
Formatted abstract
Pasteurella multocida is a Gram-negative multispecies pathogen and the causative agent of fowl cholera, a serious disease of poultry which can present in both acute and chronic forms. The major outer membrane component lipopolysaccharide (LPS) is both an important virulence factor and a major immunogen. Our previous studies determined the LPS structures expressed by different P. multocida strains and revealed that a number of strains belonging to different serovars contain the same LPS biosynthesis locus but express different LPS structures due to mutations within glycosyltransferase genes. In this study, we report the full LPS structure of the serovar 4 type strain, P1662, and reveal that it shares the same LPS outer core biosynthesis locus, L3, with the serovar 3 strains P1059 and Pm70. Using directed mutagenesis, the role of each glycosyltransferase gene in LPS outer core assembly was determined. LPS structural analysis of 23 Australian field isolates that contain the L3 locus revealed that at least six different LPS outer core structures can be produced as a result of mutations within the LPS glycosyltransferase genes. Moreover, some field isolates produce multiple but related LPS glycoforms simultaneously, and three LPS outer core structures are remarkably similar to the globo series of vertebrate glycosphingolipids. Our in-depth analysis showing the genetics and full range of P. multocida lipopolysaccharide structures will facilitate the improvement of typing systems and the prediction of the protective efficacy of vaccines.
Keyword Fowl Cholera
Human Glycosphingolipids
Structural-Analysis
Forssman Antigen
Identification
Virulence
Glycosyltransferases
Lipooligosaccharides
Diversity
Chickens
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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