A paramagnetic-reporter two-particle system for amplification-free detection of DNA in serum

Thomson, David A. C. and Cooper, Matthew A. (2013) A paramagnetic-reporter two-particle system for amplification-free detection of DNA in serum. Biosensors and Bioelectronics, 50 499-501. doi:10.1016/j.bios.2013.06.062


Author Thomson, David A. C.
Cooper, Matthew A.
Title A paramagnetic-reporter two-particle system for amplification-free detection of DNA in serum
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
1873-4235
Publication date 2013-12
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.bios.2013.06.062
Volume 50
Start page 499
End page 501
Total pages 3
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Collection year 2014
Language eng
Formatted abstract
Quantitative nucleic acid detection is used extensively in the management of many pathogenic infections, where viral or bacterial nucleic acid copy number relates directly to disease prognosis. Temperature-cycle or isothermal amplification formats offer excellent performance, but their requirement for purified nucleic acid and accurate temperature control increases costs and renders their migration to resource-limited environments problematic. In contrast, amplification-free nucleic acid assays could allow simplified system design, resulting in reduced costs. In this study, we report a amplification-free herpes simplex virus (HSV) assay where oligoethylene glycol methacrylate (OEGMA) grafted ssDNA capture-probes on paramagnetic nanoparticles are coupled with reporter-probe-modified fluorescent nanoparticles in a target-dependent manner. Following assay and reagent optimization, a sub-pM (25. amol) limit of detection could be achieved in buffer and also in neat, undiluted serum, representing a 160-fold improvement over that achieved using convention detection with a fluorescence plate reader. Equivalent performance in serum and buffer offers the opportunity for simplified diagnostic device design for resource-limited environments.
Keyword Polymer brush
Click chemistry
Diagnostic
Amplification free
Microfluidic
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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