Dysregulation of the complement cascade in the hSOD1(G93A) transgenic mouse model of amyotrophic lateral sclerosis

Lee, John D., Kamaruzaman, Nur A., Fung, Jenny N. T., Taylor, Stephen M., Turner, Bradley J., Atkin, Julie D., Woodruff, Trent M. and Noakes, Peter G. (2013) Dysregulation of the complement cascade in the hSOD1(G93A) transgenic mouse model of amyotrophic lateral sclerosis. Journal of Neuroinflammation, 10 . doi:10.1186/1742-2094-10-119


Author Lee, John D.
Kamaruzaman, Nur A.
Fung, Jenny N. T.
Taylor, Stephen M.
Turner, Bradley J.
Atkin, Julie D.
Woodruff, Trent M.
Noakes, Peter G.
Title Dysregulation of the complement cascade in the hSOD1(G93A) transgenic mouse model of amyotrophic lateral sclerosis
Journal name Journal of Neuroinflammation   Check publisher's open access policy
ISSN 1742-2094
Publication date 2013-09
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1742-2094-10-119
Open Access Status DOI
Volume 10
Total pages 14
Place of publication London, United Kingdom
Publisher BioMed Central Ltd
Collection year 2014
Language eng
Formatted abstract
Background
Components of the innate immune complement system have been implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS); however, a comprehensive examination of complement expression in this disease has not been performed. This study therefore aimed to determine the expression of complement components (C1qB, C4, factor B, C3/C3b, C5 and CD88) and regulators (CD55 and CD59a) in the lumbar spinal cord of hSOD1G93A mice during defined disease stages.

Methods
hSOD1G93A and wild-type mice were examined at four different ages of disease progression. mRNA and protein expression of complement components and regulators were examined using quantitative PCR, western blotting and ELISA. Localisation of complement components within lumbar spinal cord was investigated using immunohistochemistry. Statistical differences between hSOD1G93A and wild-type mice were analysed using a two-tailed t-test at each stage of disease progression.

Results
We found several early complement factors increased as disease progressed, whilst complement regulators decreased; suggesting overall increased complement activation through the classical or alternative pathways in hSOD1G93A mice. CD88 was also increased during disease progression, with immunolocalisation demonstrating expression on motor neurons and increasing expression on microglia surrounding the regions of motor neuron death.

Conclusions
These results indicate that local complement activation and increased expression of CD88 may contribute to motor neuron death and ALS pathology in the hSOD1G93A mouse. Hence, reducing complement-induced inflammation could be an important therapeutic strategy to treat ALS.

Keyword Motor neuron disease
Neuroinflammation
Motor Neuron Degeneration
Regulatory T Lymphocytes
Alzheimers disease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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