Chromosome 7 aneuploidy in clear cell and papillary renal cell carcinoma: Detection using silver in situ hybridization technique

Pailoor, Jayalakshmi, Rajandram, Retnagowri, Yap, Ning Yi, Ng, Keng Lim, Wang, Zhiqiang and Iyengar, Krishnan R. (2013) Chromosome 7 aneuploidy in clear cell and papillary renal cell carcinoma: Detection using silver in situ hybridization technique. Indian Journal of Pathology and Microbiology, 56 2: 98-102. doi:10.4103/0377-4929.118688


Author Pailoor, Jayalakshmi
Rajandram, Retnagowri
Yap, Ning Yi
Ng, Keng Lim
Wang, Zhiqiang
Iyengar, Krishnan R.
Title Chromosome 7 aneuploidy in clear cell and papillary renal cell carcinoma: Detection using silver in situ hybridization technique
Formatted title
Chromosome 7 aneuploidy in clear cell and papillary renal cell carcinoma: Detection using silver in situ hybridization technique
Journal name Indian Journal of Pathology and Microbiology   Check publisher's open access policy
ISSN 0377-4929
0974-5130
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.4103/0377-4929.118688
Open Access Status DOI
Volume 56
Issue 2
Start page 98
End page 102
Total pages 5
Place of publication Maharastra, India
Publisher Medknow Publications and Media
Collection year 2014
Language eng
Formatted abstract
Background: Chromosome 7 aberrations in renal cell carcinoma (RCC) have been reported in papillary renal cell carcinoma (pRCC) and clear cell renal cell carcinoma (ccRCC). However, the implication of these anomalies on prognosis and survival is still unclear. RCC Chromosome 7 aberrations have commonly been detected by fluorescent in situ hybridization and chromogenic in situ hybridization but not silver in situ hybridization (SISH). Aim: The purpose was to report chromosome 7 aberrations in ccRCC and pRCC using SISH in paraffin-embedded tissues and determine the association between the anomalies with clinical and pathological features. Materials and Methods: Cases of ccRCC and pRCC from University Malaya Medical Centre (2001-2009) were analyzed. Chromosome 7 staining was performed using an automated SISH method and association tests between chromosomal anomalies, clinical features and survival were performed. Results: SISH is a feasible technique to detect chromosome 7 aberration in RCC. Chromosome 7 aberrations with nuclear grading, staging and survival yielded no significant correlation. Surprisingly, there was a significant association between gender and chromosome 7 expressions. Though grade did not reach statistical significance for survival in our RCC cases, there was a significant correlation between overall survival with race and stage. Conclusion: Chromosome 7 aberrations in ccRCC showed no prognostic significance. Nevertheless, staging and grading systems that include prognostic variables could hold better promise.
Keyword Chromosome 7
Monosomy
Polysomy
Renal cell carcinoma
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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