Granule shearing enables the high resolution microbial identity

Lu, Y., Slater, F., Bello Mendoza, R., Jensen, P., Hugenholtz, P. and Batstone, D. J. (2013). Granule shearing enables the high resolution microbial identity. In: Juan M. Lema, Fernando Fdez-Polanco, Marta Carballa, Jorge Rodríguez and Sonia Suárez, Proceedings of 13th World Congress on Anaerobic Digestion: Recovering (bio) Resources for the World. 13th World Congress on Anaerobic Digestion (AD 13), Santiago de Compostela, Spain, (1-4). 25-28 June, 2013.

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Name Description MIMEType Size Downloads
Author Lu, Y.
Slater, F.
Bello Mendoza, R.
Jensen, P.
Hugenholtz, P.
Batstone, D. J.
Title of paper Granule shearing enables the high resolution microbial identity
Conference name 13th World Congress on Anaerobic Digestion (AD 13)
Conference location Santiago de Compostela, Spain
Conference dates 25-28 June, 2013
Proceedings title Proceedings of 13th World Congress on Anaerobic Digestion: Recovering (bio) Resources for the World
Place of Publication London, United Kingdom
Publisher International Water Association (IWA)
Publication Year 2013
Sub-type Fully published paper
Open Access Status
ISBN 9788469577561
Editor Juan M. Lema
Fernando Fdez-Polanco
Marta Carballa
Jorge Rodríguez
Sonia Suárez
Start page 1
End page 4
Total pages 4
Collection year 2014
Language eng
Formatted Abstract/Summary
Granular biomass forms naturally in high-rate anaerobic treatment systems. It is very important for relevant industrial wastewater treatment systems, which generate methane as renewable chemical energy. The granules themselves are macroscopic aggregates, with a high density and a layered structure. Understanding drivers for spatial and microbial community structure can provide opportunities to enhance this technology. Until now, phylogenetic analysis has been studies either by sectioning and microscopic analysis with fluorescent in situ hybridization, or by whole population molecular analysis by bulk methods. This means different functional and spatial layers cannot be analysed separately by bulk molecular methods, including next generation sequencing techniques. In this work, we describe a method to successfully remove microbes selectively from successive spatial layers. The removed layers can then be analysed by whole molecular methods such as pyrosequencing, and compared to in situ analysis via cryosectioning and FISH. Particularly distinct and dominant (mainly bacterial) outer layer populations can be identified through both molecular analysis on sheared off layers and in situ analysis on the whole granules.
Keyword Anaerobic granule
Shear
UASB
Granule layer
Cryosection-FISH
Q-Index Code E1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Presented during the Session "Short-presentations: Biomolecular tools" as Paper SPB01. Published as Paper IWA-12120.

Document type: Conference Paper
Collections: Official 2014 Collection
Advanced Water Management Centre Publications
 
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Created: Fri, 08 Nov 2013, 16:14:31 EST by Jon Swabey on behalf of Advanced Water Management Centre