Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis

Sewelam, Nasser, Kazan, Kemal, Thomas-Hall, Skye R., Kidd, Brendan N., Manners, John M. and Schenk, Peer M. (2013) Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis. PLoS One, 8 8: e70289.1-e70289.13. doi:10.1371/journal.pone.0070289


Author Sewelam, Nasser
Kazan, Kemal
Thomas-Hall, Skye R.
Kidd, Brendan N.
Manners, John M.
Schenk, Peer M.
Title Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Formatted title
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-08
Sub-type Article (original research)
DOI 10.1371/journal.pone.0070289
Open Access Status DOI
Volume 8
Issue 8
Start page e70289.1
End page e70289.13
Total pages 13
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2014
Language eng
Abstract Reactive oxygen species (ROS) are produced in plant cells in response to diverse biotic and abiotic stresses as well as during normal growth and development. Although a large number of transcription factor (TF) genes are up- or down-regulated by ROS, currently very little is known about the functions of these TFs during oxidative stress. In this work, we examined the role of ERF6 (ETHYLENE RESPONSE FACTOR6), an AP2/ERF domain-containing TF, during oxidative stress responses in Arabidopsis. Mutant analyses showed that NADPH oxidase (RbohD) and calcium signaling are required for ROS-responsive expression of ERF6. erf6 insertion mutant plants showed reduced growth and increased H2O2 and anthocyanin levels. Expression analyses of selected ROS-responsive genes during oxidative stress identified several differentially expressed genes in the erf6 mutant. In particular, a number of ROS responsive genes, such as ZAT12, HSFs, WRKYs, MAPKs, RBOHs, DHAR1, APX4, and CAT1 were more strongly induced by H2O2 in erf6 plants than in wild-type. In contrast, MDAR3, CAT3, VTC2 and EX1 showed reduced expression levels in the erf6 mutant. Taken together, our results indicate that ERF6 plays an important role as a positive antioxidant regulator during plant growth and in response to biotic and abiotic stresses.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
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