Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties

Braff, William A., Willner, Dana, Hugenholtz, Philip, Rabaey, Korneel and Buie, Cullen R. (2013) Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties. PLoS ONE, 8 1: e76751.1-e76751.7. doi:10.1371/journal.pone.0076751


Author Braff, William A.
Willner, Dana
Hugenholtz, Philip
Rabaey, Korneel
Buie, Cullen R.
Title Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-10-16
Sub-type Article (original research)
DOI 10.1371/journal.pone.0076751
Open Access Status DOI
Volume 8
Issue 1
Start page e76751.1
End page e76751.7
Total pages 7
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2014
Language eng
Formatted abstract
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 25 Oct 2013, 10:54:33 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences