The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster

Mayhew, Mark Leonard and Merritt, David John (2013) The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster. Arthropod Structure and Development, 42 5: 385-393. doi:10.1016/j.asd.2013.07.002


Author Mayhew, Mark Leonard
Merritt, David John
Title The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster
Formatted title
The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster
Journal name Arthropod Structure and Development   Check publisher's open access policy
ISSN 1467-8039
1873-5495
Publication date 2013-09
Sub-type Article (original research)
DOI 10.1016/j.asd.2013.07.002
Volume 42
Issue 5
Start page 385
End page 393
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2014
Language eng
Formatted abstract
Sperm storage in female insects is important for reproductive success and sperm competition. In Drosophila melanogaster females, sperm viability during storage is dependent upon secretions produced by spermathecae and parovaria. Class III dermal glands are present in both structures. Spermathecal glands are initially comprised of a three-cell unit that is refined to a single secretory cell in the adult. It encapsulates an end-apparatus joining to a cuticular duct passing secretions to the spermathecal lumen. We have examined spermatheca morphogenesis using DIC and fluorescence microscopy. In agreement with a recent study, cell division ceases by 36h after puparium formation (APF). Immunostaining of the plasma membrane at this stage demonstrates that gland cells wrap around the developing end-apparatus and each other. By 48-60h APF, the secretory cell exhibits characteristic adult morphology of an enlarged nucleus and extracellular reservoir. A novel finding is the presence of an extracellular reservoir in the basal support cell that is continuous with the secretory cell reservoir. Some indication of early spermathecal gland formation is evident in the division of enlarged cells lying adjacent to the spermathecal lumen at 18h APF and in cellular processes that bind clusters of cells between 24 and 30h APF.
Keyword Drosophila melanogaster
Spermathecae
Class III dermal glands
Development
End-apparatus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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