Mesenchymal stem cells and nano-structured surfaces

Zhou, Yinghong, Chakravorty, Nishant, Xiao, Yin and Gu, Wenyi (2013). Mesenchymal stem cells and nano-structured surfaces. In Turksen, K (Ed.), Stem Cell Nanotechnology: Methods and Protocols (pp. 133-148) New York, NY, United States: Humana Press. doi:10.1007/7651_2013_30


Author Zhou, Yinghong
Chakravorty, Nishant
Xiao, Yin
Gu, Wenyi
Title of chapter Mesenchymal stem cells and nano-structured surfaces
Title of book Stem Cell Nanotechnology: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/7651_2013_30
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627035705
9781627035712
ISSN 1064-3745
1940-6029
Editor Turksen, K
Volume number 1058
Chapter number 11
Start page 133
End page 148
Total pages 16
Total chapters 13
Collection year 2014
Language eng
Formatted Abstract/Summary
Mesenchymal stem cells (MSCs) represent multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), and adipocytes (fat cells). Their multi-potency provides a great promise as a cell source for tissue engineering and cell-based therapy for many diseases, particularly bone diseases and bone formation. To be able to direct and modulate the differentiation of MSCs into the desired cell types in situ in the tissue, nanotechnology is introduced and used to facilitate or promote cell growth and differentiation. These nano-materials can provide a fine structure and tuneable surface in nanoscales to help the cell adhesion and promote the cell growth and differentiation of MSCs. This could be a dominant direction in future for stem cells based therapy or tissue engineering for various diseases. Therefore, the isolation, manipulation, and differentiation of MSCs are very important steps to make meaningful use of MSCs for disease treatments. In this chapter, we have described a method of isolating MSC from human bone marrow, and how to culture and differentiate them in vitro. We have also provided research methods on how to use MSCs in an in vitro model and how to observe MSC biological response on the surface of nano-scaled materials.
Keyword MSCs
Cell differentiation
Bone formation
Scaffold
Nano-structured surfaces
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 11 Oct 2013, 12:24:43 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology