Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice

Tan, H. Y., Huang, L., Simmons, D., Veldhuis, J. D., Steyn, F. J. and Chen, C. (2013) Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice. Journal of Neuroendocrinology, 25 10: 910-919. doi:10.1111/jne.12078


Author Tan, H. Y.
Huang, L.
Simmons, D.
Veldhuis, J. D.
Steyn, F. J.
Chen, C.
Title Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice
Journal name Journal of Neuroendocrinology   Check publisher's open access policy
ISSN 0953-8194
1365-2826
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1111/jne.12078
Volume 25
Issue 10
Start page 910
End page 919
Total pages 10
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Collection year 2014
Language eng
Subject 1310 Endocrinology
2712 Endocrinology, Diabetes and Metabolism
2807 Endocrine and Autonomic Systems
2804 Cellular and Molecular Neuroscience
Abstract The age-associated decline in growth hormone (GH) secretion may be a consequence of the reduction in the number of GH-releasing hormone (GHRH) positive neurones. However, it remains unclear whether an alteration in the number or distribution of somatostatin (SST) neurones contributes to this change. In the present study, we characterised the role of SST in modulating the change in pulsatile GH secretion in male C57Bl/6J mice throughout puberty and into early adulthood. We assessed pulsatile GH secretion in mice at 4, 8 and 16 weeks of age. These ages correspond to early pubertal, early adulthood and adulthood, respectively. We show an elevation in peak, total and pulsatile GH secretion coinciding with periods of rapid linear growth. Using in situ hybridisation and morphometric methods, we mapped the distribution of Sst mRNA expression within the mouse brain relative to this change in pulsatile GH secretion. The results obtained show that altered pulsatile GH secretion in male mice from 4-16 weeks of age does not coincide with a significant change in the number of Sst mRNA expressing neurones or an abundance of Sst mRNA expression throughout the arcuate nucleus (ARC) and periventricular nucleus (PeV). Rather, we observed a progressive decline in Sst mRNA expressing neurones within subnuclei of the paraventricular nucleus at this time. We conclude that structural changes in Sst expression within the PeV and ARC may not reflect the observed decline in pulsatile GH secretion in mice from puberty into early adulthood.
Keyword Growth hormone
Hypothalamus
Mouse
Pubertal
Somatostatin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biomedical Sciences Publications
 
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Created: Thu, 10 Oct 2013, 00:20:22 EST by Anthony Yeates on behalf of School of Biomedical Sciences