Regulation of Helicoverpa armigera ecdysone receptor by miR-14 and its potential link to baculovirus infection

Jayachandran, Balachandran, Hussain, Mazhar and Asgari, Sassan (2013) Regulation of Helicoverpa armigera ecdysone receptor by miR-14 and its potential link to baculovirus infection. Journal of Invertebrate Pathology, 114 2: 151-157. doi:10.1016/j.jip.2013.07.004


Author Jayachandran, Balachandran
Hussain, Mazhar
Asgari, Sassan
Title Regulation of Helicoverpa armigera ecdysone receptor by miR-14 and its potential link to baculovirus infection
Formatted title
Regulation of Helicoverpa armigera ecdysone receptor by miR-14 and its potential link to baculovirus infection
Journal name Journal of Invertebrate Pathology   Check publisher's open access policy
ISSN 0022-2011
1096-0805
Publication date 2013-10
Sub-type Article (original research)
DOI 10.1016/j.jip.2013.07.004
Volume 114
Issue 2
Start page 151
End page 157
Total pages 7
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2014
Language eng
Formatted abstract
Highlights
H. armigera miRNAs were differentially expressed following HaSNPV infection.
• miR-14 and its target gene, ecdysone receptor, were down-regulated.
In vivo and in vitro analysis showed that miR-14 positively regulates the target.
• By down-regulating miR-14, HaSNPV optimises its replication.

Baculoviruses are well known for their ability to suppress the host development by altering its physiological and morphological changes. During its pathogenesis, the virus targets the host immune system, steroid hormones or apoptotic genes to evade anti-viral responses. Recent evidence indicate that viruses may use their own or host-encoded microRNAs (miRNA) to achieve this. In this study, utilizing microarray analysis, we found that the miRNA profile of Helicoverpa armigera larvae alters upon H. armigera single nucleopolyhedrovirus (HaSNPV) infection. Northern blot analyses revealed down-regulation of four miRNAs including the well-conserved and abundantly expressed miR-14. It was established that miR-14 positively regulates the H. armigera ecdysone receptor (Ha-EcR). Consistent with decreased levels of miR-14 following HaSNPV infection, the transcript levels of its target gene Ha-EcR declined around 72 h post-infection. In vivo and in vitro studies revealed that in the presence of the synthetic miR-14 mimic the transcript levels of Ha-EcR significantly increased. The miRNA–target interaction was further confirmed by a GFP reporter assay. Further, application of the miRNA mimic led to reduced replication of the virus. These results suggest that, by down-regulating miR-14, HaSNPV may reduce the Ha-EcR transcript levels providing another classic example of virus-mediated manipulation of host physiology.
Keyword MicroRNA
Baculovirus
HaSNPV
Ecdysone receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 09 Oct 2013, 10:39:17 EST by Prof Sassan Asgari on behalf of School of Biological Sciences