Flow cytometry of neural cells

Osborne, Geoffrey W. (2013). Flow cytometry of neural cells. In Brent A. Reynolds and Loic P. Deleyrolle (Ed.), Neural Progenitor Cells: Methods and Protocols (pp. 135-144) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-574-3_12


Author Osborne, Geoffrey W.
Title of chapter Flow cytometry of neural cells
Title of book Neural Progenitor Cells: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Article (original research)
DOI 10.1007/978-1-62703-574-3_12
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627035736
9781627035743
ISSN 1064-3745
1940-6029
Editor Brent A. Reynolds
Loic P. Deleyrolle
Volume number 1059
Chapter number 12
Start page 135
End page 144
Total pages 10
Total chapters 18
Collection year 2014
Language eng
Formatted Abstract/Summary
Flow cytometry is an advanced group of techniques for counting and quantifying microscopic particles such as cells, chromosomes, or functionalized beads. These approaches employ sophisticated optical and fluidic components to detect scattered light and fluorescent signals from cells as they sequentially pass an interrogation point. Cytometry plays a crucial role in the diagnosis of immunological disorders and cancers, and is a mainstay technique in basic research settings such as hematology, cell biology, and biomolecular screening. However, in spite of the breadth of applications spanning many fields, flow cytometry in neuroscience has been largely unexploited and has seen only a steady increase in interest until recent years. This is rather surprising as the potential of flow cytometry in neuroscience applications was recognized in the early 1980s as the technology was evolving.
Keyword Flow cytometry
Neural cells
Fluorescence
Imaging
Antibody
Fluorochrome
Isotype
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 02 Oct 2013, 11:47:27 EST by Jon Swabey on behalf of Queensland Brain Institute