Culturing and expansion of precursor cells from the adult hippocampus

Jhaveri, Dhanisha J., Prosper, Boris W. and Bartlett, Perry F. (2013). Culturing and expansion of precursor cells from the adult hippocampus. In Brent A. Reynolds and Loic P. Deleyrolle (Ed.), Neural Progenitor Cells: Methods and Protocols (pp. 41-51) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-574-3_4

Author Jhaveri, Dhanisha J.
Prosper, Boris W.
Bartlett, Perry F.
Title of chapter Culturing and expansion of precursor cells from the adult hippocampus
Title of book Neural Progenitor Cells: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Article (original research)
DOI 10.1007/978-1-62703-574-3_4
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627035736
ISSN 1064-3745
Editor Brent A. Reynolds
Loic P. Deleyrolle
Volume number 1059
Chapter number 4
Start page 41
End page 51
Total pages 12
Total chapters 18
Collection year 2014
Language eng
Formatted Abstract/Summary
It is now well established that a resident population of neural precursor cells continues to generate new neurons in the adult hippocampus throughout life. Numerous studies have suggested that these newborn neurons preferentially participate in the functional hippocampal circuitry that leads to enhancement of learning, cognition and mood. Therefore, understanding the molecular mechanisms that regulate the activity of these endogenous precursor cells is paramount to develop novel regenerative strategies for the treatment of neurological and psychiatric disorders. The neurosphere assay has been instrumental in discovering the presence of stem and precursor cell population from several brain regions. In this chapter, we describe this assay to specifically isolate and culture neural stem and precursor cell populations from the adult hippocampus of mice. In addition, we provide methods to conduct detailed assays to examine their functional properties such as proliferation, self-renewal, and differentiation.
Keyword Hippocampus
Neural precursor cells
Neural stem cells
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This is a protocol.

Document type: Book Chapter
Collections: Non HERDC
Queensland Brain Institute Publications
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Created: Wed, 02 Oct 2013, 11:30:06 EST by Jon Swabey on behalf of Queensland Brain Institute