Distribution of neural precursor cells in the adult mouse brain

Blackmore, Daniel G. and Rietze, Rodney L. (2013). Distribution of neural precursor cells in the adult mouse brain. In Brent A. Reynolds and Loic P. Deleyrolle (Ed.), Neural progenitor cells: methods and protocols (pp. 183-194) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-574-3_16

Author Blackmore, Daniel G.
Rietze, Rodney L.
Title of chapter Distribution of neural precursor cells in the adult mouse brain
Title of book Neural progenitor cells: methods and protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Article (original research)
DOI 10.1007/978-1-62703-574-3_16
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627035736
ISSN 1064-3745
Editor Brent A. Reynolds
Loic P. Deleyrolle
Volume number 1059
Chapter number 16
Start page 183
End page 194
Total pages 12
Total chapters 18
Collection year 2014
Language eng
Formatted Abstract/Summary
Since its inception in 1992 [Reynolds and Weiss, Science 255:1707–10, 1992], the neurosphere assay (NSA) has proven an exceptionally useful tool in detecting neural stem cells (NSCs) in both the developing and adult mammalian brain. To date, over 1,300 manuscripts have been published employing the assay, attesting to the robustness of the assay, and its ease of use. However, a brief survey of the literature demonstrates that the number of primary neurospheres generated from essentially the same anatomical region (i.e., the periventricular region of the rostral lateral ventricle) ranges between 150 and 936 [Gritti et al., J Neurosci 22:437–445, 2002; Tropepe et al., J Neurosci 17:7850–59, 1997; Doetsch et al., Cell 97:703–16, 1999; Enwere et al., J Neurosci 24:8354–65, 2004]. Indeed, in our hands we typically generate approximately 1,800 primary spheres when harvesting tissue from the same region.
Keyword Neural stem cell
Progenitor cell
Vibratome section
Subventricular zone
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This is a protocol.

Document type: Book Chapter
Collections: Non HERDC
Queensland Brain Institute Publications
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Created: Wed, 02 Oct 2013, 10:01:22 EST by Debra McMurtrie on behalf of Queensland Brain Institute