Identification of different types of respiratory neurones in the dorsal brainstem nucleus tractus solitarius of the rat

Subramanian, Hari H., Chow, Chin Moi and Balnave, Ron J. (2007) Identification of different types of respiratory neurones in the dorsal brainstem nucleus tractus solitarius of the rat. Brain Research, 1141 119-132. doi:10.1016/j.brainres.2007.01.013


Author Subramanian, Hari H.
Chow, Chin Moi
Balnave, Ron J.
Title Identification of different types of respiratory neurones in the dorsal brainstem nucleus tractus solitarius of the rat
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
1872-6240
Publication date 2007-04
Sub-type Article (original research)
DOI 10.1016/j.brainres.2007.01.013
Volume 1141
Start page 119
End page 132
Total pages 14
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract In Nembutal anaesthetised, spontaneously breathing rats, stereotaxic mapping of the nucleus tractus solitarius (NTS) for respiratory neuronal activity was undertaken. Eight different types of respiratory cells were found between 0.25 and 1.5 mm lateral to midline, extending 0.5 mm caudal to 1.5 mm rostral to obex, and 0.4-1.5 mm below the dorsal surface. A study of the respiratory motor (diaphragm EMG) and neuronal responses to excitatory amino acid (EAA) stimulation of the NTS areas was undertaken. Electrical stimulation of the vagus nerve was employed to study the NTS cellular responses to activation of pulmonary afferents. The effects of chemical activation of the midbrain periaqueductal grey (PAG) on NTS respiratory neuronal activity were investigated. EAA microinjections into the ventrolateral NTS rostral to the obex resulted in an increase in respiratory motor frequency along with increases to inspiratory cell discharge, whilst microinjections into the medial NTS caudal to the obex caused respiratory depression. EAA stimulation of calamus scriptorius produced apnea. NTS inspiratory neurones were inhibited following stimulation of ipsilateral vagus nerve, suggesting their involvement in the Hering-Breuer reflex pathway. PAG stimulation caused excitation of the NTS inspiratory cells indicating the presence of an excitatory respiratory pathway between the two nuclei. Following β-adrenergic antagonist pre-treatment of ventrolateral NTS, EAA microinjections into PAG did not evoke a cardiorespiratory effect. Based on the various findings the role of NTS in organising respiration in the rat is discussed.
Keyword Respiratory neurones
Respiratory facilitation
Apnea
Vagal escape
Beta-adrenoceptors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Queensland Brain Institute Publications
 
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Created: Tue, 01 Oct 2013, 14:54:02 EST by Hari Subramanian on behalf of UQ Centre for Clinical Research