Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle

Jayasinghe, Isuru D. and Launikonis, Bradley S. (2013) Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle. Journal of Cell Science, 126 17: 4048-4058. doi:10.1242/jcs.131565

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Author Jayasinghe, Isuru D.
Launikonis, Bradley S.
Title Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 0021-9533
Publication date 2013-09-01
Sub-type Article (original research)
DOI 10.1242/jcs.131565
Open Access Status File (Publisher version)
Volume 126
Issue 17
Start page 4048
End page 4058
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2014
Language eng
Formatted abstract
Skeletal muscle fibres are very large and elongated. In response to excitation there must be a rapid and uniform release of Ca2+ throughout for contraction. To ensure a uniform spread of excitation throughout the fibre to all the Ca2+ release sites, the muscle internalizes the plasma membrane, to form the tubular (t-) system. Hence the t-system forms a complex and dense network throughout the fibre that is responsible for excitation–contraction coupling and other signalling mechanisms. However, we currently do not have a very detailed view of this membrane network because of limitations in previously used imaging techniques to visualize it. In this study we serially imaged fluorescent dye trapped in the t-system of fibres from rat and toad muscle using the confocal microscope, and deconvolved and reconstructed these images to produce the first three-dimensional reconstructions of large volumes of the vertebrate t-system. These images showed complex arrangements of tubules that have not been described previously and also allowed the association of the t-system with cellular organelles to be visualized. There was a high density of tubules close to the nuclear envelope because of the close and parallel alignment of the long axes of the myofibrils and the nuclei. Furthermore local fluorescence intensity variations from sub-resolution tubules were converted to tubule diameters. Mean diameters of tubules were 85.9±6.6 and 91.2±8.2 nm, from rat and toad muscle under isotonic conditions, respectively. Under osmotic stress the distribution of tubular diameters shifted significantly in toad muscle only, with change specifically occurring in the transverse but not longitudinal tubules.
Keyword Tubular system
Transverse tubules
Adult guinea-pig
Frog sartorius
Reversible vacuolation
Stereological analysis
Confocal microscopy
Sarcotubular system
Cardiac myocyte
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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