Genome scale analysis of differential mRNA expression of Helicoverpa zea insect cells infected with a H. armigera baculovirus

Nguyen, Quan, Chan, Leslie C. L., Nielsen, Lars K. and Reid, Steven (2013) Genome scale analysis of differential mRNA expression of Helicoverpa zea insect cells infected with a H. armigera baculovirus. Virology, 444 1-2: 158-170. doi:10.1016/j.virol.2013.06.004


Author Nguyen, Quan
Chan, Leslie C. L.
Nielsen, Lars K.
Reid, Steven
Title Genome scale analysis of differential mRNA expression of Helicoverpa zea insect cells infected with a H. armigera baculovirus
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
Publication date 2013-09
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.virol.2013.06.004
Volume 444
Issue 1-2
Start page 158
End page 170
Total pages 13
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2014
Language eng
Formatted abstract
Knowledge of baculovirus-insect host interactions at a genome-scale level is important for developing a number of baculovirus-based applications, but the gathering of such knowledge is hindered by the lack of genomic sequences in most insect hosts. In this study, expression kinetics of 24,206 Helicoverpa zea insect transcripts and 134 Helicoverpa armigera nucleopolyhedrovirus (HearNPV) genes at 0, 12, 24 and 48. h post-infection (hpi) were simultaneously analyzed using microarrays, which were developed from sequences obtained by deep transcriptome sequencing. Host genes in pathways important for infection such as those for energy generation, anti-viral peptides, apoptosis, detoxification, DNA polymerase activities, RNA polymerase activities, translation initiation, protein processing and cell cycle arrest were identified. Differential expression was linked to changes in the number of intracellular and extracellular viral genomes and occlusion bodies. The first comprehensive elucidation of HearNPV-. H. zea expression kinetics was obtained.                                                                                                                                                                                                                                 
Keyword Baculovirus
HearNPV
Helicoverpa
Heliothis
Virus host interactions
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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