Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia

Perez-Sepulveda, Alejandra, Espana-Perrot, Pedro P., Fernandez B, Ximena, Ahumada, Veronica, Bustos, Vicente, Antonio Arraztoa, Jose, Dobierzewska, Aneta, Figueroa-Diesel, Horacio, Rice, Gregory E. and Illanes, Sebastian E. (2013) Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia. BioMed Research International, 2013 731962.1-731962.8. doi:10.1155/2013/731962

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Author Perez-Sepulveda, Alejandra
Espana-Perrot, Pedro P.
Fernandez B, Ximena
Ahumada, Veronica
Bustos, Vicente
Antonio Arraztoa, Jose
Dobierzewska, Aneta
Figueroa-Diesel, Horacio
Rice, Gregory E.
Illanes, Sebastian E.
Title Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia
Journal name BioMed Research International   Check publisher's open access policy
ISSN 2314-6133
Publication date 2013-01
Sub-type Article (original research)
DOI 10.1155/2013/731962
Open Access Status DOI
Volume 2013
Start page 731962.1
End page 731962.8
Total pages 8
Place of publication New York, NY, United States
Publisher Hindawi Publishing
Collection year 2014
Language eng
Formatted abstract
Objective: To evaluate the role of key enzymes in the methionine- homocysteine metabolism (MHM) in the physiopathology of preeclampsia (PE).

Methods: Plasma and placenta from pregnant women (32 controls and 16 PE patients) were analyzed after informed consent. Protein was quantified by western blot. RNA was obtained with RNA purification kit and was quantified by reverse transcritase followed by real-time PCR (RT-qPCR). Identification of the C677T and A1298C methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) and A2756G methionine synthase (MTR) SNP was performed using PCR followed by a high-resolution melting (HRM) analysis. S-adenosyl methionine (SAM) and S-adenosyl homocysteine (SAH) were measured in plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). The SNP association analysis was carried out using Fisher's exact test. Statistical analysis was performed using a Mann-Whitney test.

Results: RNA expression of MTHFR and MTR was significantly higher in patients with PE as compared with controls. Protein, SAM, and SAH levels showed no significant difference between preeclamptic patients and controls. No statistical differences between controls and PE patients were observed with the different SNPs studied.

Conclusion: The RNA expression of MTHFR and MTR is elevated in placentas of PE patients, highlighting a potential compensation mechanism of the methionine-homocysteine metabolism in the physiopathology of this disease.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
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