Monitoring the centrifugal recovery of recombinant protein inclusion bodies

Middelberg, A. P. J. and O'Neill, B. K. (1991) Monitoring the centrifugal recovery of recombinant protein inclusion bodies. Australian Journal of Biotechnology, 5 2: 87-89.

Author Middelberg, A. P. J.
O'Neill, B. K.
Title Monitoring the centrifugal recovery of recombinant protein inclusion bodies
Journal name Australian Journal of Biotechnology   Check publisher's open access policy
ISSN 0819-3355
Publication date 1991-04
Sub-type Article (original research)
Volume 5
Issue 2
Start page 87
End page 89
Total pages 3
Place of publication Malvern, VIC, Australia
Publisher Ausbiotech
Language eng
Formatted abstract
The industrial processing of proteins expressed as insoluble inclusion bodies employs a reasonably standard sequence of unit operations. One of these is centrifugation, which serves to concentrate the inclusion bodies after disruption of the host microorganism, and also separates the inclusion bodies from other cellular debris. Monitoring the performance of the centrifuge is essential if excessive product and hence financial loss is to be avoided and a reasonable separation obtained. The analytical disc centrifuge may be used to monitor the centrifugation. This instrument returns the sample size distribution with high resolution and without fouling. By obtaining size distributions of the centrifuge feed, supernatant and concentrate, the fractional collection efficiency of the centrifuge may be determined as a function of the Stokes diameter, and a mass balance constructed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Thu, 26 Sep 2013, 11:18:02 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology