The 37-amino-acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site

Forwood, Jade K., Brooks, Andrew, Briggs, Lyndall J., Xiao, Chong-Yun, Jans, David A. and Vasudevan, Subhash G. (1999) The 37-amino-acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site. Biochemical and Biophysical Research Communications, 257 3: 731-737. doi:10.1006/bbrc.1999.0370


Author Forwood, Jade K.
Brooks, Andrew
Briggs, Lyndall J.
Xiao, Chong-Yun
Jans, David A.
Vasudevan, Subhash G.
Title The 37-amino-acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1096-0384
Publication date 1999-04-21
Sub-type Article (original research)
DOI 10.1006/bbrc.1999.0370
Open Access Status Not Open Access
Volume 257
Issue 3
Start page 731
End page 737
Total pages 7
Collection year 2016
Language eng
Formatted abstract
The dengue virus NS5 RNA-dependent RNA polymerase has been detected in the nucleus of virus-infected mammalian cells. We demonstrate here for the first time using in vitro and in vivo assay systems that the 37-amino-acid linker interdomain of NS5 (residues 369 to 405) contains a nuclear localization sequence (NLS) which is capable of targeting b-galactosidase to the nucleus. Further, we show that the linker is recognized by subunits of the NLS-binding importin complex with an affinity similar to that of the bipartite NLS of the retinoblastoma protein and, in analogous fashion to proteins such as the SV40 large tumor antigen, contains a functional protein kinase CK2 phosphorylation site (threonine 395). Interestingly, this site appears to inhibit NS5 nuclear targeting, probably through a cytoplasmic retention mechanism. The linker may have an important role in targeting NS5 to the nucleus in a regulated manner during the dengue virus infectious cycle.
Keyword Bipartite nuclear localization sequence
Dengue virus
Importin subunits
Protein kinase CK2-phosphorylation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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