Assessing benzene-induced toxicity on wild type Euglena gracilis Z and its mutant strain SMZ

Peng, Cheng, Arthur, Dionne M., Sichani, Homa Teimouri, Xia, Qing and Ng, Jack C. (2013) Assessing benzene-induced toxicity on wild type Euglena gracilis Z and its mutant strain SMZ. Chemosphere, 93 10: 2381-2389. doi:10.1016/j.chemosphere.2013.08.037

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Author Peng, Cheng
Arthur, Dionne M.
Sichani, Homa Teimouri
Xia, Qing
Ng, Jack C.
Title Assessing benzene-induced toxicity on wild type Euglena gracilis Z and its mutant strain SMZ
Formatted title
Assessing benzene-induced toxicity on wild type Euglena gracilis Z and its mutant strain SMZ
Journal name Chemosphere   Check publisher's open access policy
ISSN 0045-6535
1879-1298
Publication date 2013-09-12
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.chemosphere.2013.08.037
Volume 93
Issue 10
Start page 2381
End page 2389
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2014
Language eng
Formatted abstract
Highlights
• Benzene induced morphological changes and lipofuscins formation in E. gracilis.
• Benzene decreased the chlorophyll content in a dose response manner.
E. gracilis SMZ is sensitive to benzene at very low concentrations (0.005–5 μM).
E. gracilis may be a suitable model for monitoring benzene in groundwater.

Benzene is a representative member of volatile organic compounds and has been widely used as an industrial solvent. Groundwater contamination of benzene may pose risks to human health and ecosystems. Detection of benzene in the groundwater using chemical analysis is expensive and time consuming. In addition, biological responses to environmental exposures are uninformative using such analysis. Therefore, the aim of this study was to employ a microorganism, Euglena gracilis (E. gracilis) as a putative model to monitor the contamination of benzene in groundwater. To this end, we examined the wild type of E. gracilis Z and its mutant form, SMZ in their growth rate, morphology, chlorophyll content, formation of reactive oxygen species (ROS) and DNA damage in response to benzene exposure. The results showed that benzene inhibited cell growth in a dose response manner up to 48 h of exposure. SMZ showed a greater sensitivity compared to Z in response to benzene exposure. The difference was more evident at lower concentrations of benzene (0.005–5 μM) where growth inhibition occurred in SMZ but not in Z cells. We found that benzene induced morphological changes, formation of lipofuscin, and decreased chlorophyll content in Z strain in a dose response manner. No significant differences were found between the two strains in ROS formation and DNA damage by benzene at concentrations affecting cell growth. Based on these results, we conclude that E. gracilis cells were sensitive to benzene-induced toxicities for certain endpoints such as cell growth rate, morphological change, depletion of chlorophyll. Therefore, it is a potentially suitable model for monitoring the contamination of benzene and its effects in the groundwater.
Keyword Benzene
Euglena gracilis
Cell growth rate
Morphology
Chlorophyll
Lipofuscin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 12 September 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
National Research Centre for Environmental Toxicology Publications
 
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Created: Mon, 23 Sep 2013, 13:05:59 EST by Dr Cheng Peng on behalf of National Res Centre For Environmental Toxicology