A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification

Liang, Fang, Arora, Neetika, Zhang, Kang Liang, Yeh, David Che Cheng, Lai, Richard, Pearson, Darnley, Barnett, Graeme, Whiley, David, Sloots, Theo, Corrie, Simon R. and Barnard, Ross T. (2013). A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification. In Dmitry M. Kolpashchikov and Yulia V. Gerasimova (Ed.), Nucleic acid detection: methods and protocols (pp. 51-68) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-535-4_4


Author Liang, Fang
Arora, Neetika
Zhang, Kang Liang
Yeh, David Che Cheng
Lai, Richard
Pearson, Darnley
Barnett, Graeme
Whiley, David
Sloots, Theo
Corrie, Simon R.
Barnard, Ross T.
Title of chapter A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification
Title of book Nucleic acid detection: methods and protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2013
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-62703-535-4_4
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627035347
9781627035354
ISSN 1064-3745
1940-6029
Editor Dmitry M. Kolpashchikov
Yulia V. Gerasimova
Volume number 1039
Chapter number 4
Start page 51
End page 68
Total pages 18
Total chapters 24
Collection year 2014
Language eng
Formatted Abstract/Summary
Quantitative real-time polymerase chain reaction (qPCR) has emerged as a powerful investigative and diagnostic tool with potential to generate accurate and reproducible results. qPCR can be designed to fulfil the four key aspects required for the detection of nucleic acids: simplicity, speed, sensitivity, and specificity. This chapter reports the development of a novel real-time multiplex quantitative PCR technology, dubbed PrimRglo™, with a potential for high-degree multiplexing. It combines the capacity to simultaneously detect many viruses, bacteria, or nucleic acids, in a single reaction tube, with the ability to quantitate viral or bacterial load. The system utilizes oligonucleotide-tagged PCR primers, along with complementary fluorophore-labelled and quencher-labelled oligonucleotides. The analytic sensitivity of PrimRglo technology was compared with the widely used Taqman® and SYBR green detection systems.
Keyword Quantitative PCR
Multiplexed PCR
Primer design
PrimRglo
Nucleic acid
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 20 Sep 2013, 09:15:07 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences