Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots

Li Dandan, Zhou Dongmei, Wang Peng, Weng Nanyan and Zhu Xiangdong (2011) Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots. Ecotoxicology and Environmental Safety, 74 4: 874-881. doi:10.1016/j.ecoenv.2010.12.006


Author Li Dandan
Zhou Dongmei
Wang Peng
Weng Nanyan
Zhu Xiangdong
Title Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots
Journal name Ecotoxicology and Environmental Safety   Check publisher's open access policy
ISSN 0147-6513
1090-2414
Publication date 2011-05
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.ecoenv.2010.12.006
Volume 74
Issue 4
Start page 874
End page 881
Total pages 8
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
The subcellular partitioning of Cd in wheat root was obtained by differential centrifugation of root homogenates to quantify the amounts of Cd associated with five operationally defined subcellular fractions, namely Cd-rich granule (MRG), cellular debris, organelles, heat-denatured protein (HDP), and heat-stable protein (HSP). Their correlations were investigated with the changes in antioxidant enzymatic activities under Cd stress (0.01-9.68. γM) for 72. h. The results showed that the relative Cd distribution in detoxified fractions (MRG+HSP) was decreased with increasing [Cd], but in metal-sensitive fractions (HDP+organelles) was increased. Consequently, the content of superoxide dismutase (SOD) and catalase (CAT) was decreased. Additionally, an increase of the total antioxidant capacity (A-TOC) and a slight decrease of malondialdehyde (MDA) were also observed. Interestingly, the relative root elongation (RRL) and CAT were significantly correlated with the Cd-sensitive fractions. These results suggest that the Cd toxicity to wheat roots strongly depends on the subcellular Cd distribution
Keyword Cadmium
Subcellular distribution
Antioxidant enzymatic activities
Wheat
Biotic Ligand Model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 19 Sep 2013, 15:47:35 EST by Peng Wang on behalf of School of Agriculture and Food Sciences